Light Microscopy

Zeiss

CFCI-WF 01 ApoTome I

Category

ApoTome, Widefield

Facility

LMF & EMF CFCI

Location

MTZ, A.20.055

Link to Booking

https://cgc.med.tu-dresden.de/phpScheduleIt/

Description

Widefield System with ApoTome This microscope is designed for routine work on fixed or living samples. Optical sectioning can be obtained on fixed samples by using the ApoTome (structured illumination). The microscope is also capable for brightfield including contrast techniques like phase contrast, darkfield and polarized light. Furthermore the second camera (colour) allows the imaging of histological stained samples.

Suitable for Specimen

  • Fixed samples
  • Live samples
  • Tissue sections

Suitable for Technique

  • Multi wellplate imaging
  • Large area tile/mosaic scans
  • Multiple positions

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    5.2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420340-9901-000
  • Immersion:
    Air
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420350-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.71 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420360-9900-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420780-9900-000

Condenser

H/DIC, Ph1, Ph2, Ph3, VAREL, NA 0.55, working distance 26 mm, manual

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (HAL 100, 12V, 5600 K)

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps
  • Port, Magnification:
    right side port
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

XY Stage

Zeiss Scanningtisch 130x85 motP; CAN (D), motorized
  • universal holder K (slides, dishes 24-68mm)
  • universal holder K-X (for multiwellplates)
  • heating insert P-Set 2000 (Pecon)

Structured Illumination

ApoTome1, structured illumination for optical sectioning

Additional Equipment

Pecon Heating Insert P-Set 2000
for dishes and slides

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Core i5-4670
  • RAM:
    2 x 8 GB
  • Graphics Card:
    NVIDIA GeForce 8500 GT
  • Display(s):
    30"

Getting started

Equipment

Publications