Light Microscopy

Zeiss

MZ2 - Zeiss 2photon inverted

Category

Multi photon, Laser scanning confocal

Facility

LMF MPI-CBG

Location

MPI-CBG, 456A

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

 Zeiss LSM 780 NLO Multiphoton Laser Scanning Microscope with a tunable pulsed near-infra-red laser (Chameleon Vision II) for multiphoton excitation. Single/multi-photon point scanning confocal system with two PMTs and one 32-channel GaAsP detector for spectral detection, two non-descanned GaAsP detectors and three PMTs for multi-photon detection, and three transmitted light detectors. Motorized stage for multi-point acquisition.

Suitable for Specimen

  • Fixed samples
  • Live samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans
  • Laser cutting/ ablation
  • Multi wellplate imaging
  • Photo manipulation (PA/FRAP/FLIP/FLAP/...)
  • Spectral unmixing

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9800-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440640-9903
  • Immersion:
    W / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0-0.17 mm
  • Working Distance:
    0.57 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 25/0,8 II (426947)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420852-9870
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.62 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421867-9970
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421767-9970
  • Immersion:
    W / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.17 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 63/1,30 II HC (426958); LCI PN 63/1,30 III (426959)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420882-9970-720
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420780-9900-000

Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, manual

Fluorescence Filter Cubes

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, manual Metal halide lamp)
  • 405nm (integrated Laser Diode)
  • 561nm (integrated Laser DPSS)
  • 594nm (integrated Laser HeNe)
  • 633nm (integrated Laser HeNe)
  • 458, 488, 514nm multiline (manual Laser Argon)
  • 680-1080 nm (Coherent, manual Laser Multiphotonen Chameleon)

XY Stage

Märzhauser SCAN IM 130x100 DC 1mm, motorized
  • universal disholder
  • multiwell plate holder
  • heating insert P S1

Hardware Autofocus

Zeiss Definite Focus.1

Scan Head

conventional scanner
Internal Detectors
  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • 2 confocal PMT (spectral)
  • T-PMT
Additional Detectors
  • 2 GaAsP (Zeiss BiG module)
  • 3 PMT (epi-detection)
  • 2 PMT (trans-detection)

Incubation

stage top incubator (Bachhofer chamber, H. Saur)

Feature
Heating

Additional Information

Getting started

Equipment

Publications