Light Microscopy
Zeiss
DZNE C1 LSM 700 (retired!!!)
Category
Laser scanning confocal
Facility
LMF DZNE
Location
DZNE,
253
Description
Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
Suitable for Specimen
- Tissue sections
- Fixed samples
Suitable for Technique
- Large area tile/mosaic scans
Microscope Stand
Zeiss
Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus
Objectives
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Immersion:Air
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Coverslip Thickness:0.17 mm
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Working Distance:2 mm
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Thread:M27
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Tube Length:∞
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Zeiss Order Number:420640-9800-000
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Immersion:Air
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Collar:coverslip correction
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Coverslip Thickness:0.13-0.21 mm
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Working Distance:0.25 mm
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Thread:M27
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Tube Length:∞
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Zeiss Order Number:420660-9970
Condenser
BF, Ph1, Ph2, Ph3, DIC,
NA 0.55, working distance 26 mm, manual
Fluorescence Filter Cubes
- G 365, excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
- BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
- LP 495, dichromatic mirror, longpass, Zeiss
- BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
- BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Transmitted Illumination
- Lamp (Halogen, 100W)
Fluorescence Illumination
- HXP 120C (120W, Zeiss, manual Metal halide lamp)
- 405nm (integrated Laser Diode)
- 488nm (integrated Laser DPSS)
- 555nm (integrated Laser DPSS)
- 639nm (integrated Laser Diode)
XY Stage
Märzhauser SCAN IM 130x100 DC 1mm, motorized
- universal disholder
Scan Head
conventional scanner
Internal Detectors
- 2 confocal PMT (filter)