Light Microscopy

Zeiss

ApoTome2 inverse CRTD

Category

ApoTome, Widefield

Facility

LMF CMCB

Location

BIOTEC, 2nd floor, 227

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php

Description

Our inverted LiveCell-ApoTome2 Microscope with incubation, hardware autofocus, colour and monochrome cameras is designed for routine work on fixed sample and living samples. Optical sectioning can be obtained on fixed samples by the use of the ApoTome2 slider.

Suitable for Specimen

  • Fixed samples
  • Tissue sections
  • Live samples

Suitable for Technique

  • Large area tile/mosaic scans
  • Multi wellplate imaging
  • Timelapse imaging
  • Multiple positions

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Additional Magnification

Optovar 1x / 1.6x, motorized

Z Focus Device Additional

PI Z-Piezo WSB 500

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    10.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441021-9910-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    5.2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420341-9911-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    7.9 mm
  • Nomarski Prism:
    LD PN 20/0,40 II (426954); PlasDIC for LD PN 20/0,40 Korr (444504)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441341-9970-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440641-9911-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420650-9901-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    1.7 mm at cover glass 0.75 mm
  • Nomarski Prism:
    LD PN 63x/0,75 II (426956); LD PN 63x/0,75 Korr (444506)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421380-9970-000

Condenser

BF, DIC I, DIC II, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, motorized

Additional Filter(s)

Zeiss excitation filter wheel (Switching Time 60 ms)
  • BP 625/50 (BP 600-650), excitation filter, bandpass
  • BP 436/25 (BP 424-449), excitation filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • LED Peaks (nm): 385, 430, 475, 545, 650, 735 (X-Cite, integrated LED)
  • 470, 590 nm (Zeiss, integrated Colibri LED)

Camera

  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12 fps at full chip size
  • Port, Magnification:
    right side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

XY Stage

PI scanning stage 130x110 PIEZO, motorized
  • fixed holder for two slides a 76x26mm
  • fixed holder for two labtek
  • insert for four 3.5 cm dishes
  • multiwell plate holder
  • multiwell plate holder with CO2 lid

Hardware Autofocus

Zeiss Definite Focus.2

Structured Illumination

ApoTome2, structured illumination for optical sectioning

Incubation

cage incubator (Pecon)

Feature
CO2, Heating, Humidity

Computer

  • OS:
    Windows 10
  • CPU:
    Intel Xeon E5-2643 (2x)
  • RAM:
    4 x 8 GB
  • Display(s):
    30"
Software Package(s)
Zeiss ZEN blue 2.6
Module(s): Advanced Processing, Autofocus, Experiment Designer, Extended Focus, Measurement, Multi channel acquisition, Software Autofocus, Tiles, Time Lapse, Z-stack

Getting started

Equipment

Publications