Light Microscopy

Zeiss

WF Slide scanner Axioscan CRTD

Category

Slide scanner, Widefield

Facility

LMF CMCB

Location

CRTD, ground floor south, -1.120

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php

Description

It is a Zeiss widefield System dedicated to screening of fixed samples on slides. It provides automated imaging in fluorescent and transmitted light and can process up to 100 slides in one cycle.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans
  • Multiple positions

Microscope Stand

Zeiss Axioscan.Z1, upright stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    12.5 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420130-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    Planapo 10/0,45 II (1045-073)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420350-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420650-9901-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 mm
  • Nomarski Prism:
    EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420660-9970-000

Condenser

BF, Ring Aperture Contrast (RAC), motorized

Fluorescence Filter Cubes

  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 676/29 (BP 663-692), emission filter, bandpass, Zeiss
  • LP 409, dichromatic mirror, longpass, Zeiss
  • BP 447/60 (BP 417-477), emission filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 514/30 (BP 499-529), emission filter, bandpass, Chroma
  • LP 573, dichromatic mirror, longpass, Zeiss
  • BP 595/31 (BP 580-611), emission filter, bandpass, Zeiss
  • BP 385/30; BP 469/38; BP 591/27; BP 735/40 (BP 370-400; BP 450-488; BP 578-605; BP 715-755), excitation filter, quadro bandpass, Zeiss
  • DM 405+493+611+762, dichromatic mirror, quadro bandpass, Zeiss
  • BP 425/30; BP 524/51; BP 634/38; BP 785/38 (BP 410-440; BP 498-549; BP 615-653; BP 766-804), emission filter, quadro bandpass, Zeiss

Additional Filter(s)

Zeiss excitation filter wheel (Switching Time 50 ms)
  • BP 385/30; BP 469/38; BP 591/27; BP 735/40 (BP 370-400; BP 450-488; BP 578-605; BP 715-755), excitation filter, quadro bandpass, Zeiss

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • 385, 430, 475, 555, 590, 630, 735 nm (Zeiss, integrated Colibri 7 LEDs)

Camera

  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    7.176 x 5.319 mm
  • Maximum Frame Size:
    1600 x 1200 dexels
  • Bit Depth:
    24 bit
  • Max Full Chip Frame Rate:
    30 fps
  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    2048 x 2048 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    100 fps

XY Stage

Zeiss Präzisions-Scanningtisch 300x100mm, motorized

Computer

  • OS:
    Windows 10
  • CPU:
    Intel Xeon E5-2643
  • RAM:
    128 GB
  • Graphics Card:
    AMD FirePro V5900 (2GB)
  • Display(s):
    30"
Software Package(s)
Zeiss ZEN 2 (blue)
Module(s): Advanced Processing, Experiment Designer, Extended Focus, Measurement, Multi channel acquisition, Software Autofocus, Tiles, Z-stack

Getting started

Equipment

Publications