Light Microscopy

ApoTome2 3rd floor upright CRTD

Category

ApoTome, Widefield

Facility

LMF CMCB

Location

, 3.325

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php

Description

It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome2.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans

Microscope Stand

Axio Imager.Z1, upright stand, manual objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    8.7 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420120-9901-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420640-9800-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420650-9901-000
  • Immersion:
    Water
  • Working Distance:
    2.1 mm
  • Nomarski Prism:
    Achroplan 40/0,75 W III (444451)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    440090
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 mm
  • Nomarski Prism:
    EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420660-9970-000
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    440668

Condenser

BF, DF, DIC II, DIC III, Ph1, Ph2, Ph3, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

  • BP 485/20; BP 578/14 (BP 475-495; BP 571-585), excitation filter, double bandpass, Zeiss
  • BP 528/65; LP 600 (BP 495-560; LP 600), double dichromatic mirror, double bandpass, Zeiss
  • BP 528/25; LP 610 (BP 515-540; LP 610), emission filter, double bandpass, Zeiss
  • BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
  • LP 400, dichromatic mirror, longpass, AHF
  • BP 460/50 (BP 435-485), emission filter, bandpass, AHF
  • BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • BP 525/36 (BP 507-543), emission filter, bandpass, AHF
  • BP 590/33 (BP 573-606), emission filter, bandpass, AHF
  • LP 565, dichromatic mirror, longpass, AHF
  • BP 545/25 (BP 533-558), excitation filter, bandpass, AHF
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss
  • BP 500/20 (BP 490-510), excitation filter, bandpass, AHF
  • LP 515, dichromatic mirror, longpass, Leica
  • BP 535/30 (BP 520-550), emission filter, bandpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps
  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

XY Stage

Märzhäuser, Typ: EK 75x50 SZ; Serial-No: 07121212, motorized
  • holder for 1 slide

Structured Illumination

ApoTome2, structured illumination for optical sectioning

Computer

  • OS:
    Windows 7 Ultimate (dual boot 32/64 bit)
  • CPU:
    Intel Xeon E5-2643 (2x)
  • RAM:
    8 x 8 GB
  • Display(s):
    2 x 24"
Software Package(s)
ZEN blue V 2012
Module(s): 3D VisArt, Experiment Designer, Extended Focus, Measurement, Multi channel acquisition, Tiles, Time Lapse, Z-stack
Axiovision

Getting started

Equipment

Publications