Light Microscopy

Zeiss

DZNE SD2 Spinning Disk 2 Incubation

Category

Spinning disk

Facility

LMF DZNE

Location

, 251

Link to Booking

https://techpool.biotec.tu-dresden.de/?scheduleid=sc14ffd3aa3e44c3

Description

This Spinning disc system comes with a dark microscope stand incubation chamber, with which you can hold a stable temperature and CO2 environment.

Suitable for Specimen

  • Fixed samples
  • Live samples

Suitable for Technique

  • Multiple positions
  • Large area tile/mosaic scans
  • Multi wellplate imaging

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    8.8 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420320-9901
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    12.1 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420630-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    5.2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420341-9911
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420660-9970
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420792-9900

Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • BP 605/70 (BP 570-640), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 550/25 (BP 538-563), emission filter, bandpass, Zeiss
Filter Cube Link
  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Filter Cube Link
  • BP 690/50 (BP 665-715), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 640/30 (BP 625-655), emission filter, bandpass, Zeiss
  • BP 370/40; BP 474/28; BP 585/35 (BP 350-390; BP 460-488; BP 568-603), excitation filter, bandpass, Zeiss
  • DM 395+495+610, triple dichromatic mirror, triple bandpass, Zeiss
  • BP 425/46; BP 527/54; LP 615 (BP 402-448; BP 500-554; LP 615), emission filter, triple bandpass, Zeiss

Additional Filter(s)

Zeiss emission filter wheel (Switching Time 30 ms)
  • BP 450/50 (BP 425-475), emission filter, bandpass, Zeiss
  • BP 617/73 (BP 581-654), emission filter, bandpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, integrated Metal halide lamp)
  • 365, 470, 590, 625 nm (Zeiss, integrated Colibri LED)
  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser OPSL)
  • 568nm (Laser DPSS)
  • 635nm (integrated Laser Diode)

Camera

  • Port, Magnification:
    SD port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

XY Stage

Märzhäuser Scanningtisch 130x100 STEP, motorized
  • universal holder K (slides, dishes 24-68mm)
  • fixed holder for 4 slides
  • multiwell plate holder
  • heating insert P S1

Hardware Autofocus

Zeiss Definite Focus.2

Incubation

cage incubator (Zeiss)

Feature
CO2, Heating

Additional Information

Getting started

Equipment

Publications