Light Microscopy

ApoTome2 inverse BIOTEC

Category

ApoTome, Widefield

Facility

LMF CMCB

Location

, 225

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php

Description

Our Apotome microscope inverted is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome2.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans
  • Multi wellplate imaging

Microscope Stand

Axiovert 200M, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    8.7 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420120-9901-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    10.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    441021-9910-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    Planapo 10/0,45 II (1045-073)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    000000-1063-139
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    440640-9903-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 mm
  • Nomarski Prism:
    EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    440654-9902-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    Planapo 63x/1,40 III (1122-909)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    000000-1113-108
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.11 mm
  • Nomarski Prism:
    alpha Plan-Fluar 100/1,45 III (1131-718)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    000000-1084-514

Condenser

BF, DIC III, Ph1, Ph2, Ph3, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
  • LP 400, dichromatic mirror, longpass, Zeiss
  • BP 460/50 (BP 435-485), emission filter, bandpass, Zeiss
  • BP 545/25 (BP 533-558), excitation filter, bandpass, Chroma
  • LP 565, dichromatic mirror, longpass, Chroma
  • BP 605/70 (BP 570-640), emission filter, bandpass, Chroma
  • BP 625/50 (BP 600-650), excitation filter, bandpass
  • LP 665, dichromatic mirror, longpass
  • BP 690/50 (BP 665-715), emission filter, bandpass
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    front port, 0.6x
  • Sensor Size:
    8.9 x 6.7 mm
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps
  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

XY Stage

Märzhäuser, SCAN IM, S/N 03111993, motorized

Structured Illumination

ApoTome2, structured illumination for optical sectioning

Computer

  • OS:
    Windows 7 Ultimate (dual boot 32/64 bit)
  • CPU:
    Intel Xeon E5-2643 (2x)
  • RAM:
    8 x 8 GB
  • Graphics Card:
    AMD FirePro V5900 (2GB)
  • Display(s):
    30"
Software Package(s)
ZEN blue V2012 1.1.2.0
Module(s): Experiment Designer, Extended Focus, Image Analysis, Measurement, Multi channel acquisition, Tiles, Time Lapse, Z-stack
Axiovision

Getting started

Equipment

Publications