Light Microscopy

Zeiss

Live Cell 4D BIOTEC

Facility

LMF CMCB

Location

BIOTEC, 2nd floor, 226

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?sid=2 _blank - "BIOTEC/CRTD Booking System"

Description

Our Zeiss Axiovert 200 4D-System is designed for rapid acquisition LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. Objectives can be mounted on a piezo for very fast z-acquisition.

Suitable for Specimen

Live samples
Fixed samples
Tissue sections

Suitable for Technique

Multi wellplate imaging
Timelapse imaging
3D imaging
Multiple positions

Microscope Stand

Zeiss Axiovert 200M, inverted stand, motorized objective revolver, motorized z focus

Objectives

5x/0.15 Air Ph1 EC Plan-Neofluar

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

13.6 mm
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440321-9901-000

10x/0.3 Air Ph1 EC Plan-Neofluar

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

5.2 mm
Nomarski Prism:

EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440331-9902-000

10x/0.45 Air DIC Plan-Apochromat

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

2 mm
Nomarski Prism:

Planapo 10/0,45 II (1045-073)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

000000-1063-139

20x/0.3 Air Ph2 EC Plan-Neofluar

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

2 mm
Nomarski Prism:

EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440341-9904-000

20x/0.75 Air Ph2 EC Plan-Neofluar

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

0.71 mm
Nomarski Prism:

EC PN 40/0,75 II; 100/1,30III (426945)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440351-9903-000

20x/0.8 Air Ph2 Plan-Apochromat

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

0.55 mm
Nomarski Prism:

EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440641-9911-000

20x/0.8 Air DIC Plan-Apochromat

Immersion:

Air
Coverslip Thickness:

0.17 mm
Working Distance:

0.55 mm
Nomarski Prism:

EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440640-9903-000

40x/0.6 Air Ph2 LD Plan-Neofluar

Immersion:

Air
Collar:

coverslip correction
Coverslip Thickness:

0.17 mm
Working Distance:

2.9 mm
Nomarski Prism:

LD PN 40/0,60 II (426955); PlasDIC for LD PN 40/0,60 Korr (444505)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

441351-9970-000

40x/0.6 Air DIC LD Plan-Neofluar

Immersion:

Air
Collar:

coverslip correction
Coverslip Thickness:

0.17 mm
Working Distance:

2.9 mm at cover glass 0.75 mm
Nomarski Prism:

LD PN 40/0,60 II (426955); LD PN 40/0,6 (444505)
Thread:

M27
Tube Length:

∞
Zeiss Order Number:

421360-9970-000

40x/1.3 Oil Ph3 EC Plan-Neofluar

Immersion:

Oil
Coverslip Thickness:

0.17 mm
Working Distance:

0.2 mm
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

440451-9903-000

40x/1.3 Oil DIC Plan-Neofluar

Immersion:

Oil
Coverslip Thickness:

0.17 mm
Working Distance:

0.21 mm
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

1056-602 (1083-997)

63x/1.2 Water DIC C-Apochromat

Immersion:

Water
Collar:

coverslip correction
Coverslip Thickness:

0.17 mm
Working Distance:

0.28 mm
Nomarski Prism:

EC PN 63/1,25 III; CA 63/1,2W III (426946); EC PN HC 63/1,25 II; CA HC 63/1,20 W II (426950)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

441777-9970-000

63x/1.4 Oil Ph3 Plan-Apochromat

Immersion:

Oil
Coverslip Thickness:

0.17 mm
Working Distance:

0.19 mm
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

1056-602 (1083-997)

100x/1.4 Oil DIC Plan-Apochromat

Immersion:

Oil
Coverslip Thickness:

0.17 mm
Working Distance:

0.17 mm
Nomarski Prism:

PA 100/1,4 HC II (426949); PA 40x/1,30 III (426965)
Thread:

RMS (20.32×0.71 mm)
Tube Length:

∞
Zeiss Order Number:

000000-1113-108

Condenser

BF, DIC II, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

motorized revolver

Cy5 (Alexa633/Cy5) - Zeiss set 49006

BP 620/60 (BP 590-650), excitation filter, bandpass, Chroma
LP 660, dichromatic mirror, longpass, Chroma
BP 700/75 (BP 663-738), emission filter, bandpass, Chroma

polarizer (DIC/Polarization) - Zeiss set Analysator

GFP (FITC, Alexa488/GFP) - Zeiss set 49002

BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
LP 495, dichromatic mirror, longpass, Chroma
BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

calcium imaging (Calcium Imaging) - Zeiss

BP 340/12; BP 380/14, excitation filter, double bandpass
LP 405, dichromatic mirror, longpass
BP 510/40, emission filter, bandpass

DAPI (DAPI, Alexa 350) - Zeiss

BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
LP 400, dichromatic mirror, longpass, Leica
BP 460/50 (BP 435-485), emission filter, bandpass, AHF

GFP (FITC, Alexa488/GFP) - Zeiss

LP 405, dichromatic mirror, longpass
BP 510/40 (BP 490-530), emission filter, bandpass

CFP/YFP - Zeiss set 59217

BP 430/24; BP 500/20 (BP 418-442; BP 490-510), excitation filter, double bandpass, Chroma
BP 433/22; BP 500/18 (BP 422-444; BP 492-510), double dichromatic mirror, double bandpass, Chroma
BP 469/22; BP 535/30 (BP 458-480; BP 520-550), emission filter, double bandpass, Chroma

GFP/RFP - Zeiss set 59022

BP 470/40; BP 572/35 (BP 450-490; BP 555-590), excitation filter, double bandpass, Chroma
BP 522/52; BP 637/83 (BP 496-548; BP 595-678), double dichromatic mirror, double bandpass, Chroma
BP 522/41; BP 632/62 (BP 501-542; BP 601-663), emission filter, double bandpass, Chroma

GFP (FITC, Alexa488/GFP) - Zeiss set F36-497

BP 472/30 (BP 457-487), excitation filter, bandpass, AHF
LP 495, dichromatic mirror, longpass, AHF
LP 496, emission filter, longpass, AHF

Fura ratio imaging (calcium) (Calcium Imaging) - Zeiss set F76-520

BP 340/26 (BP 327-353), excitation filter, bandpass, AHF
BP 387/11 (BP 385-392), excitation filter, bandpass, AHF
LP 409, dichromatic mirror, longpass, AHF
BP 510/84 (BP 468-552), emission filter, bandpass, AHF

Texas red (DsRed, mCherry/TexasRed) - Zeiss set F46-008

BP 560/40 (BP 540-580), excitation filter, bandpass, AHF
LP 585, dichromatic mirror, longpass, AHF
BP 630/75 (BP 593-668), emission filter, bandpass, AHF

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

DG4 (Sutter, integrated Xenon lamp)
Spectra X Light engine (Lumencor, integrated LED)

Camera

Hamamatsu Orca Flash 4.0 V3, Monochrome sCMOS camera, dexel size 6.5 µm

Port, Magnification:

left side port, 1x
Sensor Size:

13.312 x 13.312 mm
Maximum Frame Size:

2048 x 2048 dexels
Bit Depth:

16 bit
Max Full Chip Frame Rate:

100 fps

XY Stage

Prior, Model: H117N2X2/B, SN: 53607, motorized

Incubation

cage incubator (Pecon)

Feature
CO₂, Heating, Humidity

Computer

Lynx (LiveCell-4D-BIO)

OS:

Windows 7 Professional (64 bit)
CPU:

Intel Core i7-4770
RAM:

2 x 8 GB
Graphics Card:

Intel HD Grafik 4600
Display(s):

2 x 24"

Getting started

Equipment

Publications