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Light Microscopy

Leica

LSI Macro Confocal CRTD

Category

Single Photon, Laser scanning confocal

Facility

LMF CMCB

Location

CRTD, basement south, -1.120

Description

It is an upright super zoom macro confocal microscope with a tuneable spectral detector. It has an 16x optical zoom. The systems offers 4 laser lines and a spectral detector. It has a large working distance and a field of view up to 16mm.

Suitable for Specimen

  • Fixed samples

Microscope Stand

Leica DMRE, upright stand, manual objective revolver, motorized z focus

Additional Magnification

16x optical zoom, motorized

Z Focus Device Additional

Leica SuperZ stage

Objectives

  • Immersion:
    Air
  • Tube Length:
  • Leica Order Number:
    10447176
  • Immersion:
    Air
  • Tube Length:
  • Leica Order Number:
    10447243

Fluorescence Filter Cubes

  • BP 537/45 (BP 515-560), excitation filter, bandpass, Leica
  • LP 585, dichromatic mirror, longpass, Leica
  • LP 590, emission filter, longpass, Leica
  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica
  • BP 390/70 (BP 355-425), excitation filter, bandpass, Leica
  • LP 455, dichromatic mirror, longpass, Leica
  • LP 470, emission filter, longpass, Leica
  • BP 472/30 (BP 457-487), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • LP 496, emission filter, longpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Leica, integrated Metal halide lamp)
  • 405nm pulsed (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 532nm (integrated Laser HeNe)
  • 635nm (integrated Laser Diode)

XY Stage

Leica LSI mot. Scanning Stage Kit m. LSTEP, motorized
  • multiwell plate holder
  • variable holder for slides and 3.5cm dishes

Scan Head

conventional scanner
Internal Detectors
  • 1 spectral detection PMT
  • T-PMT

Computer

  • OS:
    Windows XP Professional
  • CPU:
    Intel Core i7-860
  • RAM:
    2 x 2 GB
  • Display(s):
    2 x 19"
Software Package(s)
Leica LAS AF
Module(s): Acquisition, Base, Live Data Mode
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