Light Microscopy

Leica

Live Cell AF6000 CRTD

Category

Widefield

Facility

LMF CMCB

Location

CRTD, basement south, -1.123

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?&

Description

Our Leica AFLX6000 is designed for routine work and dedicated for LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. The simple LASAF interface makes it straight forward to work with. A double micromanipulator allows precise manipulation of the sample.

Suitable for Specimen

  • Live samples
  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Multi wellplate imaging
  • Timelapse imaging
  • Multiple positions

Microscope Stand

Leica DMI 6000, inverted stand, motorized objective revolver, motorized z focus

Additional Magnification

Optovar 1x / 1.6x, motorized

Objectives

  • Immersion:
    Oil
  • Collar:
    NA correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Nomarski Prism:
    D
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506251

Condenser

BF, DIC I, DIC II, NA 0.55, working distance 28 mm, motorized

Fluorescence Filter Cubes

  • BP 628/40 (BP 608-648), excitation filter, bandpass, Chroma
  • LP 660, dichromatic mirror, longpass, AHF
  • BP 692/40 (BP 672-712), emission filter, bandpass, AHF
  • BP 360/40 (BP 340-380), emission filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica
  • BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
  • LP 505, dichromatic mirror, longpass, Leica
  • BP 527/30 (BP 512-542), emission filter, bandpass, Leica
  • BP 537/45 (BP 515-560), excitation filter, bandpass, Leica
  • LP 580, dichromatic mirror, longpass, Leica
  • LP 590, emission filter, longpass, Leica
  • BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • LP 500, emission filter, longpass, AHF
  • BP 500/20 (BP 490-510), excitation filter, bandpass, AHF
  • LP 515, dichromatic mirror, longpass, Leica
  • BP 535/30 (BP 520-550), emission filter, bandpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Leica, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    bottom port
  • Sensor Size:
    8.2 x 8.2 mm
  • Maximum Frame Size:
    512 x 512 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    38.5 fps
  • Port, Magnification:
    left side port
  • Sensor Size:
    9 x 6.7 mm
  • Maximum Frame Size:
    1392 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    21.07 - 10.54 fps

XY Stage

Märzhäuser EK 127x83 mot, SN: 07082835, motorized

Incubation

cage incubator (Pecon)

Feature
CO2, Heating, Humidity

Computer

  • OS:
    Windows XP Professional
  • CPU:
    Intel Core 2 Duo E6400
  • RAM:
    2 x 1 GB
  • Display(s):
    2 x 24"
Software Package(s)
Leica LAS AF
Module(s): 3D, Acquisition, Base, Dye Finder, Live Data Mode

Getting started

Equipment

Publications