Light Microscopy

Zeiss

LSM 780 upright CRTD

Category

Laser scanning confocal

Facility

LMF CMCB

Location

CRTD, basement south, -1.123

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?&

Description

It is an upright Laser Scanning Confocal microscope with 32 spectral detection channels (GaAsP), 2 PMTs, one Airyscan-detector and one transmission PMT. A selection of objective lenses is listed below, but more are available, too.
Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, spectral unmixing of overlapping emission spectra, Airyscan super resolution. It can acquire brightfield and DIC images with the transmission detector.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans
  • Spectral unmixing

Microscope Stand

Zeiss Axio Imager.Z1, upright stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    Planapo 10/0,45 II (1045-073)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9900-000
  • Immersion:
    Water
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    1.8 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440638-9901-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420650-9901-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420650-9901-000
  • Immersion:
    Water
  • Working Distance:
    2.5 mm
  • Nomarski Prism:
    W PA 40/1,0 III (426967-9010)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421462-9900-000
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.28 mm
  • Nomarski Prism:
    CA 40/1,2 W; LD CA 40/1,1 W III (426948)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421767-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Nomarski Prism:
    CA 40/1,2 W; LD CA 40/1,1 W III (426948)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420762-9900-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.11 mm
  • Nomarski Prism:
    PA 100/1,46 III (426963)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420792-9800-000

Condenser

H/DIC, DIC II, DIC III, DF, Ph1, Ph2, Ph3, NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

  • BP 347/55 (BP 320-375), excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 545/25 (BP 533-558), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, integrated Metal halide lamp)
  • 405nm (integrated Laser Diode)
  • 458, 488, 514nm multiline (integrated Laser Argon)
  • 561nm (integrated Laser DPSS)
  • 594nm (integrated Laser HeNe)
  • 633nm (integrated Laser HeNe)

XY Stage

Wienecke&Sinske GmbH, Typ: Scanningtisch M-686K008, SN: 11-05111018022, motorized

Scan Head

conventional scanner
Internal Detectors
  • 2 confocal PMT (spectral)
  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • AiryScan
  • T-PMT

Computer

  • OS:
    Windows 7 Ultimate (64 bit)
  • CPU:
    Intel Xeon E5-2643
  • RAM:
    48 GB
  • Display(s):
    30"
Software Package(s)
Zeiss ZEN black 2.1 V 11.0.0.190
Module(s): 3D, AiryScan, FCS Basis, FCS Extended, FCS PCH, FRAP, Tiles, VME

Getting started

Equipment

Publications