Light Microscopy

Zeiss

LSM 700 inverse BIOTEC

Category

Laser scanning confocal

Facility

LMF CMCB

Location

BIOTEC, 2nd floor, 227

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?&

Description

It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Multi wellplate imaging
  • Large area tile/mosaic scans

Microscope Stand

Zeiss Axiovert 200M, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    Planapo 10/0,45 II (1045-073)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440640-9903-000
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Nomarski Prism:
    CA 40/1,2 W; LD CA 40/1,1 W III (426948)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441757-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.21 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1056-602 (1083-997)
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420782-9900-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949); PA 40x/1,30 III (426965)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420792-9900-000

Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 480/30 (BP 465-495), excitation filter, bandpass
  • LP 505, dichromatic mirror, longpass
  • BP 535/40 (BP 515-555), emission filter, bandpass
  • BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
  • LP 400, dichromatic mirror, longpass, AHF
  • BP 460/50 (BP 435-485), emission filter, bandpass, AHF
  • BP 545/10 (BP 540-550), excitation filter, bandpass
  • LP 555, dichromatic mirror, longpass
  • BP 600/70 (BP 565-635), emission filter, bandpass

Additional Filter(s)

Zeiss emission filter wheel
  • SP 490, emission filter, shortpass, Zeiss
  • SP 555, emission filter, shortpass, Zeiss
  • SP 640, emission filter, shortpass, Zeiss
  • BP 523/65 (BP 490-555), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, manual Metal halide lamp)
  • 405nm pulsed (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 555nm (integrated Laser DPSS)
  • 639nm (integrated Laser DPSS)

XY Stage

Märzhäuser Scan IM 130x100 DC, Art-No: 90-25-422-000/432028-9901, SN: 11020881, motorized

Scan Head

conventional scanner
Internal Detectors
  • 2 confocal PMT (filter)
  • T-PMT

Computer

  • OS:
    Windows 7 Ultimate (64 bit)
  • CPU:
    Intel Core 2 Duo E8400
  • RAM:
    4 x 2 GB
  • Graphics Card:
    ATI FirePro V5800 (1GB)
  • Display(s):
    30"
Software Package(s)
Zeiss ZEN black 2011 V 8.1.0.484 SP3
Module(s): 3D, FRAP, Tiles

Getting started

Equipment

Publications