Light Microscopy

LSM 780/FCS inverse BIOTEC

Category

Laser scanning confocal

Facility

LMF CMCB

Location

, 221

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?&

Description

It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides F(C)CS functionality by the ConfoCor3 module. For FCS please ask Markus Burkhardt.

Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.

Suitable for Specimen

  • Fixed samples
  • Tissue sections
  • Molecules at the surface of a cover slip
  • Live samples

Suitable for Technique

  • Large area tile/mosaic scans
  • Spectral unmixing

Microscope Stand

Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    Planapo 10/0,45 II (1045-073)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420640-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420650-9901-000
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.28 mm
  • Nomarski Prism:
    CA 40/1,2 W; LD CA 40/1,1 W III (426948)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    421767-9970-711
  • Immersion:
    W / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    LCI PN 63/1,30 II HC (426958); LCI PN 63/1,30 III (426959)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420882-9970-720

Condenser

BF, DIC II, DIC III, working distance 26 mm, manual

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
  • BP 545/25 (BP 533-558), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
  • BP 387/11 (BP 381-392), excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, manual Metal halide lamp)
  • 405nm (integrated Laser Diode)
  • 458, 488, 514nm multiline (integrated Laser Argon)
  • 561nm (integrated Laser DPSS)
  • 633nm (integrated Laser HeNe)

XY Stage

Märzhäuser Scan IM 130x100 DC, Art-No: 90-25-422-0000/432028-9901, SN: 10042885, motorized

Scan Head

Incubation

splitable cage incubator

Feature
CO2, Heating, Humidity

Additional Equipment

MBSPort
Plateinvisible
MBS -405invisible
MBS -445invisible
MBS T80/R20invisible
MBS 514/561visible
MBS 458visible
MBS 458/514visible
MBS 458/514/561/633visible
MBS 488visible
MBS 488/561visible
MBS 488/633visible
MBS 488/561/633visible
MBS T80/R20visible
Platevisible

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Xeon E5-2643 (2x)
  • RAM:
    8 x 8 GB
  • Graphics Card:
    AMD FirePro V5900 (2GB)
  • Display(s):
    30"
Software Package(s)
ZEN black 2011 V 11.0.1.190 test123
Module(s): 3D, FCS Basis, FCS Extended, FCS PCH, FRAP, RICS, Tiles, VME

Getting started

Equipment

Publications