Light Microscopy

LSM 780/FLIM inverse CRTD

Category

Laser scanning confocal

Facility

LMF CMCB

Location

, -1.124

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?&

Description

It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.

Suitable for the detection of multiple fluorescent dyes (but no DAPI), FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.

Suitable for Specimen

  • Fixed samples
  • Tissue sections
  • Live samples

Suitable for Technique

  • Large area tile/mosaic scans
  • Spectral unmixing
  • FLIM

Microscope Stand

Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420650-9901-000
  • Immersion:
    Water
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    1.1 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420967-9970-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 mm
  • Nomarski Prism:
    EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420660-9970-000
  • Immersion:
    W / Si / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.41 mm at cover glass 0.17
  • Nomarski Prism:
    LD LCI PApo 63/1,20 III (426947)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420862-9970-799
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420782-9900-000

Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 545/30 (BP 530-560), excitation filter, bandpass, Chroma
  • LP 570, dichromatic mirror, longpass, Chroma
  • BP 620/60 (BP 590-650), emission filter, bandpass, Chroma
  • BP 436/20 (BP 426-446), excitation filter, bandpass, AHF
  • LP 455, dichromatic mirror, longpass, AHF
  • BP 480/40 (BP 460-500), emission filter, bandpass, AHF
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
  • BP 347/55 (BP 320-375), excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • LP 565, dichromatic mirror, longpass, AHF
  • BP 525/50 (BP 500-550), emission filter, bandpass, AHF
  • BP 617/73 (BP 581-654), emission filter, bandpass, AHF
  • DM Polarization, dichromatic mirror, longpass, AHF
  • BP 525/50 (BP 500-550), emission filter, bandpass, AHF
  • LP 498, dichromatic mirror, longpass, AHF
  • BP 479/40 (BP 459-499), emission filter, bandpass, AHF
  • BP 535/22 (BP 524-546), emission filter, bandpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, manual Metal halide lamp)
  • 440nm pulsed (Laser Diode)
  • 473nm, pulsed (integrated Laser Diode)
  • 458, 488, 514nm multiline (integrated Laser Argon)
  • 561nm (integrated Laser DPSS)
  • 633nm (integrated Laser HeNe)

XY Stage

Märzhäuser Scan IM 130x100 DC, Art-No: 90-25-422-0000, SN: 12080329, motorized

Scan Head

Incubation

splitable cage incubator

Feature
CO2, Heating, Humidity

Additional Equipment

FLIM dual channel unit from B&H

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Xeon E5-2623 v3
  • RAM:
    1 x 16 GB
  • Display(s):
    30"
Software Package(s)
ZEN black 2011 V 8.0.7.273
Module(s): 3D, FCS Basis, FCS Extended, FCS PCH, FRAP, RICS, Tiles, VME

Getting started

Equipment

Publications