Light Microscopy

Leica

SP5 I upright BIOTEC

Category

Laser scanning confocal

Facility

LMF CMCB

Location

BIOTEC, 2nd floor, 220

Link to Booking

https://techpool.biotec.tu-dresden.de/index.php?redirect=%2Fschedule.php%3F%26

Description

Our Leica TCS SP5 I confocal setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Suitable for Specimen

  • Live samples
  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Spectral unmixing

Microscope Stand

Leica DM 6000, upright stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Leica SuperZ stage

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.2 mm
  • Nomarski Prism:
    A
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506284
  • Immersion:
    Water
  • Working Distance:
    3.5 mm
  • Nomarski Prism:
    D1
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506147
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.59 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506166
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.28 mm
  • Nomarski Prism:
    D
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506173
  • Immersion:
    Water
  • Working Distance:
    3.3 mm
  • Nomarski Prism:
    D1
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506155
  • Immersion:
    Water
  • Working Distance:
    2.2 mm
  • Nomarski Prism:
    D1
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506148
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.22 mm
  • Nomarski Prism:
    D
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506213
  • Immersion:
    Oil
  • Collar:
    NA correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Nomarski Prism:
    E
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506192
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506372
  • Immersion:
    Oil
  • Collar:
    temperature and coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.09 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506318

Condenser

BF, DIC I, DIC II, DIC III, NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

  • BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
  • LP 505, dichromatic mirror, longpass, Leica
  • BP 527/30 (BP 512-542), emission filter, bandpass, Leica
  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica
  • LP 570, dichromatic mirror, longpass, AHF
  • BP 620/60 (BP 590-650), emission filter, bandpass, AHF
  • BP 545/30 (BP 530-560), excitation filter, bandpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Leica, integrated Metal halide lamp)
  • 405nm pulsed (integrated Laser Diode)
  • 458, 477, 488, 496, 514nm multiline (integrated Laser Argon)
  • 561nm (integrated Laser DPSS)
  • 633nm (integrated Laser HeNe)

XY Stage

Märzhäuser EK 14 mot. 14C DM 600, SN: 05021155, motorized

Scan Head

conventional scanner
Internal Detectors
  • T-PMT
  • 2 spectral detection HyD
  • 3 confocal PMT

Incubation

splitable cage incubator (Life Imaging Service)

Feature
CO2, Heating, Humidity

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Xeon E5-2650
  • RAM:
    4 x 4 GB
  • Graphics Card:
    NVIDIA QUADRO 600
  • Display(s):
    30"
Software Package(s)
Leica LAS AF V 2.7.3
Module(s): 3D, Acquisition, Base, Colocalization, Dye Finder, FRAP, FRET, Live Data Mode

Getting started

Equipment

Publications