Light Microscopy

Zeiss

WF Laser Microdissection CRTD

Category

Microdissection, Widefield

Facility

LMF CMCB

Location

CRTD, basement south, -1.121

Link to Booking

https://techpool.biotec.tu-dresden.de/index.php?redirect=%2Fschedule.php%3F%26

Description

It is a laser microdissection system consisting of an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color)

Suitable for the manipulation of biological samples with a UV laser and the collection of cut material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working imaging system.

Suitable for Specimen

  • Tissue sections

Suitable for Technique

  • Laser cutting/ ablation

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    12.5 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420130-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    5.2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420340-9901-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    7.9 mm at cover glass 0.75 mm
  • Nomarski Prism:
    LD PN 20/0,40 II (426954); LD PN 40/0,6 (444505)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421351-9970-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.9 mm at cover glass 0.75 mm
  • Nomarski Prism:
    LD PN 40/0,60 II (426955); LD PN 40/0,6 (444505)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421360-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.21 mm
  • Nomarski Prism:
    EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440654-9902-000
  • Immersion:
    Glycerine
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm at cover glass 0.16 mm
  • Nomarski Prism:
    PA 150x/1,35 III (426968-9000)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420792-9970-000

Condenser

BF, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 510, dichromatic mirror, longpass, Zeiss
  • LP 515, emission filter, longpass, Zeiss
  • BP 367/11 (BP 362-373), excitation filter, bandpass, AHF
  • LP 405, dichromatic mirror, longpass, AHF
  • BP 447/60 (BP 417-477), emission filter, bandpass, AHF
  • BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
  • LP 488, dichromatic mirror, longpass, AHF
  • BP 525/50 (BP 500-550), emission filter, bandpass, AHF
  • BP 524/25 (BP 512-537), excitation filter, bandpass, AHF
  • LP 561, dichromatic mirror, longpass, AHF
  • BP 605/70 (BP 570-640), emission filter, bandpass, AHF
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 635, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, integrated Metal halide lamp)
  • 355nm (Laser Diode)

Camera

  • Port, Magnification:
    left side port
  • Sensor Size:
    6.3 x 4.8 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    8 bit
  • Max Full Chip Frame Rate:
    15.38 fps

XY Stage

Robo RoboStage II, motorized

Photo Manipulation

Zeiss Laser microdissection (PALM)

Photo Manipulation Modality
Scanner

Computer

  • OS:
    Windows 7 Ultimate (64 bit)
  • CPU:
    Intel Xeon E5-2643
  • RAM:
    4 x 4 GB
  • Graphics Card:
    AMD FirePro V4900
  • Display(s):
    21" LCD Pen + 24"
Software Package(s)
Zeiss Axiovision

Getting started

Equipment

Publications