Light Microscopy

Leica

WF Leica inverse CRTD

Category

Widefield

Facility

LMF CMCB

Location

, 225

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?&

Description

The inverted Leica microscope is designed for routine work on fixed fluorescent sample.

The instrument is especially suitable for multicolor fluorescent samples due to high sensitivity b/w camera.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Multi wellplate imaging

Microscope Stand

Leica DMI 4000, inverted stand, manual objective revolver, motorized z focus

Z Focus Device Additional

Leica SuperZ stage

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    13.7 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506224
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    10 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506289
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    6.9 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506242
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.3 - 1.9 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    11506201

Condenser

BF, DIC I, DIC II, working distance 28 mm, motorized

Fluorescence Filter Cubes

  • BP 490/20 (BP 480-500), excitation filter, bandpass, Leica
  • LP 515, dichromatic mirror, longpass, Leica
  • BP 535/30 (BP 520-550), emission filter, bandpass, Leica
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
  • BP 436/20 (BP 426-446), excitation filter, bandpass, AHF
  • LP 455, dichromatic mirror, longpass, AHF
  • BP 480/40 (BP 460-500), emission filter, bandpass, AHF
  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica
  • BP 545/25 (BP 533-558), excitation filter, bandpass, AHF
  • LP 565, dichromatic mirror, longpass, AHF
  • BP 605/70 (BP 570-640), emission filter, bandpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Leica, integrated Metal halide lamp)

Camera

  • Sensor Size:
    9 x 6.7 mm
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    21.07 - 10.54 fps

XY Stage

Leica, fixed stage, manual

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Core Quad Q6700
  • RAM:
    4 GB
  • Graphics Card:
    NVIDIA QUADRO FX1700
  • Display(s):
    2 x 19"
Software Package(s)
Leica LAS AF V 4.0.0

Getting started

Equipment

Publications