Light Microscopy

OLYMPUS

WF Olympus upright BIOTEC

Category

Widefield

Facility

LMF CMCB

Location

BIOTEC, 2nd floor, 227

Link to Booking

https://techpool.biotec.tu-dresden.de/index.php?redirect=%2Fschedule.php%3F%26

Description

Our Olympus videomicroscope upright is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to the use of two possible cameras, a b/w Photometrics and a color Spot CCD. Timelapse, along with z-stacks can be performed with the use of motorized z-stage.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • 3D imaging

Microscope Stand

OLYMPUS BX 61, upright stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Prior NanoScanZ

Objectives

  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    17 mm
  • Nomarski Prism:
    10x/0.30W II (444438)
  • Thread:
    M27
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    1234
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.1 mm
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.18 mm
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    N1478700
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.15 mm
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949)
  • Thread:
    M27
  • Tube Length:
    ∞

Condenser

BF, DF, DIC I, DIC II, DIC III, Ph1, Ph2, Ph3, NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

  • BP 480/20 (BP 470-490), excitation filter, bandpass
  • LP 495, dichromatic mirror, longpass
  • BP 525/50 (BP 500-550), emission filter, bandpass
  • BP 545/25 (BP 533-558), excitation filter, bandpass, Chroma
  • LP 565, dichromatic mirror, longpass, Chroma
  • BP 605/70 (BP 570-675), emission filter, bandpass, Chroma
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
  • BP 365/10 (BP 360-370), excitation filter, bandpass, OLYMPUS
  • LP 400, dichromatic mirror, longpass, OLYMPUS
  • LP 420, emission filter, longpass, OLYMPUS
  • BP 540/20 (BP 530-550), excitation filter, bandpass, OLYMPUS
  • LP 570, dichromatic mirror, longpass, OLYMPUS
  • LP 590, emission filter, longpass, OLYMPUS
  • BP 620/60 (BP 590-650), excitation filter, bandpass, AHF
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HBO (100W, Mercury arc lamp)

Camera

  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    8.7 x 6.9 mm
  • Maximum Frame Size:
    1300 x 1030 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    15.38 fps
  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    15.2 x 15.2 mm
  • Maximum Frame Size:
    2048 x 2048 dexels
  • Bit Depth:
    14 bit
  • Max Full Chip Frame Rate:
    5 fps
  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    8.7 x 6.6 mm
  • Maximum Frame Size:
    4080 x 3072 dexels
  • Bit Depth:
    12 bit

XY Stage

Olympus U-UCD8A, SN: 1E05676, manual
  • fixed holder for 4 slides

Computer

  • OS:
    Windows XP Professional
  • CPU:
    Intel Pentium 4
  • RAM:
    2 x 1 GB + 2 x 0.5 GB
  • Graphics Card:
    NVIDIA QUADRO FX540
  • Display(s):
    19"
Software Package(s)
Molecular Devices MetaXpress
Molecular Devices MetaMorph
Module(s): 3D
Diagnoscics Instruments SPOT Basic Software V 5.1 5678

Getting started

Equipment

Publications