Light Microscopy

WF Screening System BIOTEC

Category

High throughput microscope, Widefield

Facility

LMF CMCB

Location

, 242

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?sid=2

Description

t is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates.

Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed..

Suitable for Specimen

  • Fixed samples
  • Live samples

Suitable for Technique

  • Multiple positions
  • Multi wellplate imaging

Microscope Stand

ImageXpress Micro XL, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    8.5 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Order Number:
    1-6300-0451
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    20 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Order Number:
    1-6300-0121
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    16 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    4 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Order Number:
    6500-0120
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    8.2 - 6.9 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    1.0 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Order Number:
    1-6300-0196
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 - 0.16 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Order Number:
    1-6300-0412

Condenser

BF, Ph0, Ph1, manual

Transmitted Illumination

  • (Nikon 100W Pillar Diascopic Illuminator)

Fluorescence Illumination

  • Spectra X Light engine (integrated LED)

XY Stage

special stage, motorized
  • multiwell plate holder

Incubation

cage incubator (heating unit from Nikon)

Feature
Heating, CO2

Computer

  • OS:
    Windows 7
Software Package(s)
MetaXpress

Getting started

Equipment

Publications