Light Microscopy

ApoTome2 2nd floor inverse CRTD

Category

ApoTome, Widefield

Facility

LMF CMCB

Location

, 2.230

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php

Description

It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the ApoTome2.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans
  • Multi wellplate imaging
  • 3D imaging

Microscope Stand

Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    9.5 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    440310-9903-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    5.2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420341-9911-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420650-9901-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.25 mm
  • Nomarski Prism:
    EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420660-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.21 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420460-9900-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420780-9900-000

Condenser

BF, DIC II, Ph1, Ph2, Ph3, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 620/60 (BP 590-650), excitation filter, bandpass, Chroma
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
  • BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
  • BP 387/11 (BP 381-392), excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, manual Metal halide lamp)

Camera

  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

XY Stage

Märzhäuser Scan IM 130x110 DC, SN: 09052062, motorized
  • universal holder K-X (for multiwellplates)
  • universal holder K (slides, dishes 24-68mm)

Structured Illumination

ApoTome2, structured illumination for optical sectioning

Computer

  • OS:
    Windows 7 Ultimate (dual boot 32/64 bit)
  • CPU:
    Intel Xeon E5-2643 (2x)
  • RAM:
    8 x 8 GB
  • Display(s):
    2 x 24"
Software Package(s)
ZEN blue V2012
Module(s): 3D VisArt, Autofocus, Experiment Designer, Measurement, Multi channel acquisition, Tiles, Time Lapse, Z-stack
Axiovision

Additional Information

Getting started

Equipment

Publications