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Light Microscopy

Ultramicroscope CRTD

Category

Light sheet

Facility

LMF CMCB

Location

CRTD, ground floor south, -1.120

Description

Single Plane Illumination microscope Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used.

Suitable for 3D multicolor imaging of large transparent specimen.

Suitable for Specimen

  • Cleared samples

Microscope Stand

MVX10, upright stand, manual objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    20 mm

Additional Filter(s)

excitation filter wheel (Switching Time 30 ms)
  • BP 405/40 (BP 385-425), excitation filter wheel, bandpass
  • BP 470/40 (BP 450-490), excitation filter wheel, bandpass
  • BP 500/20 (BP 490-510), excitation filter wheel, bandpass
  • BP 545/60 (BP 515-575), excitation filter wheel, bandpass
  • BP 560/40 (BP 540-580), excitation filter wheel, bandpass
  • BP 620/40 (BP 600-640), excitation filter wheel, bandpass
  • BP 740/35 (BP 723-758), excitation filter wheel, bandpass

Fluorescence Illumination

  • Laser Fianium Supercontinuum SC400-4 - range 410 to 2400 nm (> 4 W, integrated Laser)

Camera

  • Port, Magnification:
    top port
  • Sensor Size:
    16.4 x 14 mm

Additional Equipment

there are 2 different dipping caps available
  • short version - longer working distance of 7 mm but less resolution
  • long version - shorter working distance and higher resolution

Computer

  • OS:
    Windows 7
  • CPU:
    Intel Core i5
  • RAM:
    16 GB
  • Display(s):
    30"
Software Package(s)
ImSpektor
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