Light Microscopy

Zeiss

W3 - Zeiss CCD Upright !! Retired !!

Category

Widefield

Facility

LMF MPI-CBG

Location

MPI-CBG, U32

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

It is an upright, motorized microscope with medium sensitivity CCD camera. For quick checking of your samples.

Suitable for Specimen

  • Fixed samples
  • Tissue sections
  • Organoids

Microscope Stand

Zeiss Axioplan 2, upright stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    12.5 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440125
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    5.2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440330
  • Immersion:
    Water
  • Working Distance:
    1.9 mm
  • Nomarski Prism:
    20/0,5W II (444448)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440049
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.71 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440350
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.29 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440060
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.17 mm
  • Working Distance:
    0.24 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440668
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440762-9904
  • Immersion:
    Oil
  • Collar:
    NA correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420496-9900
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949); PA 100/1,40 III (426941)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440782-9902
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    13.6 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440321-9901-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    5.2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440331-9902-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    Planapo 10/0,45 II (1045-073)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    000000-1063-139
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440341-9904-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.71 mm
  • Nomarski Prism:
    EC PN 40/0,75 II; 100/1,30III (426945)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440351-9903-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440640-9903-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440641-9911-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.9 mm
  • Nomarski Prism:
    LD PN 40/0,60 II (426955); PlasDIC for LD PN 40/0,60 Korr (444505)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441351-9970-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.9 mm at cover glass 0.75 mm
  • Nomarski Prism:
    LD PN 40/0,60 II (426955); LD PN 40/0,6 (444505)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421360-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.21 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1056-602 (1083-997)
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440451-9903-000
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.28 mm
  • Nomarski Prism:
    EC PN 63/1,25 III; CA 63/1,2W III (426946); EC PN HC 63/1,25 II; CA HC 63/1,20 W II (426950)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441777-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1056-602 (1083-997)
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949); PA 40x/1,30 III (426965)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    000000-1113-108

Condenser

H/DIC, Ph1, Ph2, Ph3, DF, DIC II, DIC III, NA 0.9, working distance 1.2 mm, manual

Fluorescence Filter Cubes

  • BP 390/18 (BP 381-399), excitation filter, bandpass, AHF
  • LP 416, dichromatic mirror, longpass, AHF
  • BP 460/60 (BP 430-490), emission filter, bandpass, AHF
  • BP 434/17 (BP 425-443), excitation filter, bandpass, AHF
  • LP 452, dichromatic mirror, longpass, AHF
  • BP 479/40 (BP 459-499), emission filter, bandpass, AHF
  • BP 472/30 (BP 457-487), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • BP 520/35 (BP 503-538), emission filter, bandpass, AHF
  • BP 543/22 (BP 532-554), excitation filter, bandpass, AHF
  • LP 562, dichromatic mirror, longpass, AHF
  • BP 593/40 (BP 573-613), emission filter, bandpass, AHF
  • BP 562/40 (BP 542-582), excitation filter, bandpass, AHF
  • LP 593, dichromatic mirror, longpass, AHF
  • BP 641/75 (BP 604-679), emission filter, bandpass, AHF
  • BP 628/40 (BP 608-648), excitation filter, bandpass, AHF
  • LP 660, dichromatic mirror, longpass, AHF
  • BP 692/40 (BP 672-712), emission filter, bandpass, AHF
  • BP 500/20 (BP 480-520), excitation filter, bandpass, AHF
  • LP 515, dichromatic mirror, longpass, AHF
  • BP 535/30 (BP 520-550), emission filter, bandpass, AHF

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • X-Cite 120W (X-Cite, Metal halide lamp)

Camera

  • Port, Magnification:
    top port
  • Sensor Size:
    1/3 inch
  • Maximum Frame Size:
    1024x768 dexels
  • Bit Depth:
    8 bit
  • Max Full Chip Frame Rate:
    30 fps
  • Port, Magnification:
    top port, 1x
  • Maximum Frame Size:
    1600 x 1200 dexels
  • Bit Depth:
    12 bit

XY Stage

manual xy stage, manual
  • holder for 1 slide

Additional Equipment

reflection darkfield oil condenser

Getting started

Equipment

Publications