Light Microscopy

OLYMPUS

CFCI-SD 01 Spinning Disk

Category

Spinning disk

Facility

LMF & EMF CFCI

Location

MTZ, A.20.028

Link to Booking

https://cgc.med.tu-dresden.de/phpScheduleIt/

Description

The Olympus spinning disc microscope is designed for fast "real-time" confocal imaging of living samples. Furthermore that system is especially designed for mouse intravital microscopy. The microscope is also capable for brightfield including contrast techniques like DIC, darkfield and polarized light.

Suitable for Specimen

  • Whole organs
  • Live samples
  • Fixed samples
  • Whole organisms

Suitable for Technique

  • 3D imaging
  • Timelapse imaging
  • Multiple positions
  • Large area tile/mosaic scans
  • Mouse intravital microscopy

Microscope Stand

OLYMPUS IX 83, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Olympus Fast 4D Imaging Piezo Z stage

Objectives

  • Coverslip Thickness:
    - mm
  • Working Distance:
    13 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO4X - UIS 2
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO10X2 - UIS 2
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.6 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO20X - UIS 2
  • Coverslip Thickness:
    0.13-0.19 mm
  • Working Distance:
    0.8 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO30XS
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.18 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO40X - UIS 2
  • Immersion:
    Water
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.28 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO60XW - UIS 2
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.15 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    PLAPON60XO - UIS 2

Fluorescence Filter Cubes

  • BP 470/20 (BP 460-480), excitation filter
  • LP 485, dichromatic mirror
  • BP 518/45 (BP 495-540), emission filter
  • BP 545/20 (BP 535-555), excitation filter
  • LP 565, dichromatic mirror
  • BP 598/55 (BP 570-625), emission filter
  • BP 620/60 (BP 590-650), excitation filter, bandpass, OLYMPUS
  • LP 660, dichromatic mirror, longpass, OLYMPUS
  • BP 700/75 (BP 663-738), emission filter, bandpass, OLYMPUS

Additional Filter(s)

Andor emission filter wheel (Switching Time 50 ms)

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HBO (100W, Mercury arc lamp)
  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 640nm (integrated Laser Diode)

Camera

  • Port, Magnification:
    Left side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Maximum Frame Size:
    512 x 512 dexels

XY Stage

Prior PR-IX3-STG3, motorized
  • universal disholder
  • multiwell plate holder
  • low profile slide holder (75x25mm)
  • heating insert P-Set 2000 (Pecon)
  • heating insert P (Pecon)

Hardware Autofocus

OLYMPUS

Scan Head

Incubation

(P-Set 2000 for dishes and slides, Pecon)

Feature
CO2, Heating, Humidity

Additional Equipment

Setup for mouse intravital microscopy:
  • Surgery unit with heating lamp
  • Induction box for pre-anesthesia
  • BioLite Intubation System
  • Mouse Ventilation System: Minivent (Hugo Sachs)
  • Silicone heating plate with temperature sensor (Friedrich Freek)

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Xeon E5-1620
  • RAM:
    2 GB
  • Graphics Card:
    NVIDIA QUADRO 2000
  • Display(s):
    27"

Getting started

Equipment

Publications