Light Microscopy

CFCI-SD 01 Spinning Disk

Category

Spinning disk

Facility

LMF & EMF CFCI

Location

, A.20.028

Link to Booking

https://cgc.med.tu-dresden.de/phpScheduleIt/

Description

The Olympus spinning disc microscope is designed for fast "real-time" confocal imaging of living samples. Furthermore that system is especially designed for mouse intravital microscopy. The microscope is also capable for brightfield including contrast techniques like DIC, darkfield and polarized light.

Suitable for Specimen

  • Live samples
  • Fixed samples
  • Whole organisms
  • Whole organs

Suitable for Technique

  • Multiple positions
  • Large area tile/mosaic scans
  • Mouse intravital microscopy
  • 3D imaging
  • Timelapse imaging

Microscope Stand

IX 83, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Olympus Fast 4D Imaging Piezo Z stage

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    13 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    UPLSAPO4X - UIS 2
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    UPLSAPO10X2 - UIS 2
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.6 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    UPLSAPO20X - UIS 2
  • Immersion:
    Silicone
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.19 mm
  • Working Distance:
    0.8 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    UPLSAPO30XS
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.18 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    UPLSAPO40X - UIS 2
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.28 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    UPLSAPO60XW - UIS 2
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.15 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    PLAPON60XO - UIS 2

Condenser

H/DIC, working distance 27 mm, motorized

Fluorescence Filter Cubes

  • BP 470/20 (BP 460-480), excitation filter
  • LP 485, dichromatic mirror
  • BP 518/45 (BP 495-540), emission filter
  • BP 545/20 (BP 535-555), excitation filter
  • LP 565, dichromatic mirror
  • BP 598/55 (BP 570-625), emission filter
  • BP 620/60 (BP 590-650), excitation filter, bandpass, OLYMPUS
  • LP 660, dichromatic mirror, longpass, OLYMPUS
  • BP 700/75 (BP 663-738), emission filter, bandpass, OLYMPUS

Additional Filter(s)

emission filter wheel (Switching Time 50 ms)

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HBO (100W, Mercury arc lamp)
  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 640nm (integrated Laser Diode)

Camera

  • Port, Magnification:
    Left side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    56 fps

XY Stage

Prior PR-IX3-STG3, motorized
  • universal disholder
  • multiwell plate holder
  • low profile slide holder (75x25mm)
  • heating insert P-Set 2000 (Pecon)
  • heating insert P (Pecon)

Hardware Autofocus

IX81-ZDC 2

Scan Head

  • Maximum Spinning Disk Rotation Speed: 1500 - 5000 rpm
  • Physical Pinhole Diameter: 50 µm
  • Physical Pinhole Spacing: 250 µm

Incubation

stage top incubator (P-Set 2000 for dishes and slides)

Feature
CO2, Heating, Humidity

Additional Equipment

Setup for mouse intravital microscopy:
  • Surgery unit with heating lamp
  • Induction box for pre-anesthesia
  • BioLite Intubation System
  • Mouse Ventilation System: Minivent (Hugo Sachs)
  • Silicone heating plate with temperature sensor (Friedrich Freek)

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Xeon E5-1620
  • RAM:
    2 GB
  • Graphics Card:
    NVIDIA QUADRO 2000
  • Display(s):
    27"

Getting started

Equipment

Publications