Light Microscopy

CFCI-LSM 02 Leica TCS SP5

Category

Laser scanning confocal

Facility

LMF & EMF CFCI

Location

, B.20.001C

Link to Booking

https://cgc.med.tu-dresden.de/phpScheduleIt/

Description

The Leica SP 5 confocal laserscanning microscope is designed for routine work. The Incubator and a CO2 system allow experiments with living cells. The laser lines are well distributed over the spectrum. Special techniques like FRET and FRAP are can be done easily. Due to the conventional setup, it offers straight forward working conditions. The microscope is also capable for brightfield.

Suitable for Specimen

  • Live samples
  • Fixed samples

Suitable for Technique

  • Spectral unmixing
  • 3D imaging
  • Multiple positions
  • Timelapse imaging

Microscope Stand

DMI 6000, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    13.7 mm
  • Tube Length:
    ∞
  • Order Number:
    11506224
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.2 mm
  • Tube Length:
    ∞
  • Order Number:
    11506285
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.59 mm
  • Tube Length:
    ∞
  • Order Number:
    11506513
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Tube Length:
    ∞
  • Order Number:
    11506253
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.18 mm
  • Working Distance:
    0.22 mm
  • Tube Length:
    ∞
  • Order Number:
    11506213
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Tube Length:
    ∞
  • Order Number:
    11506192

Computer

  • OS:
    Windows 7 Professional (64 bit)
  • CPU:
    Intel Xeon E5-1620 v2
  • RAM:
    8 GB
  • Graphics Card:
    NVIDIA QUADRO K600 (1GB)
  • Display(s):
    2 x 19"

Getting started

Equipment

Publications