Light Microscopy
DZNE M1 LSM 780 NLO
Category
Multi photon, Laser scanning confocal
Facility
LMF DZNE
Location
, 252
Description
Zeiss LSM 780 NLO
- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
- Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition.Suitable for Specimen
- Fixed samples
- Cleared samples
- Whole organs
- Tissue sections
Suitable for Technique
- Spectral unmixing
- 3D imaging
Microscope Stand
Axio Examiner.Z1, upright stand, manual objective revolver, motorized z focus
Objectives
-
Immersion:Air
-
Coverslip Thickness:0.17 mm
-
Working Distance:2 mm
-
Thread:M27
-
Tube Length:∞
-
Order Number:420640-9800-000
-
Immersion:Scale
-
Working Distance:3.7 mm
-
Thread:M27
-
Tube Length:∞
-
Order Number:421440-9800
-
Immersion:Scale
-
Collar:immersion correction
-
Working Distance:5.6 mm
-
Thread:M32
-
Tube Length:∞
-
Order Number:421459-9770
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Immersion:Air
-
Collar:coverslip correction
-
Coverslip Thickness:0.13-0.21 mm
-
Working Distance:0.25 mm
-
Thread:M27
-
Tube Length:∞
-
Order Number:420660-9970
Condenser
BF, DIC II, DIC III,
working distance 2 mm with 0.17mm cover glas and water immersion, manual
Fluorescence Filter Cubes
- G 365, excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
- BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
- LP 495, dichromatic mirror, longpass, Zeiss
- BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
- BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Additional Filter(s)
emission filter
- BP 592/35 (BP 575-610), emission filter, bandpass, Zeiss
- LP 488, dichromatic mirror, longpass, Zeiss
- LP 560, dichromatic mirror, longpass, Zeiss
- LP 643, dichromatic mirror, longpass, Zeiss
Transmitted Illumination
- Lamp (Halogen, 100W)
Fluorescence Illumination
- HXP 120C (120W, manual Metal halide lamp)
- 405nm (integrated Laser Diode)
- 633nm (integrated Laser HeNe)
- 561nm (integrated Laser DPSS)
- 458, 488, 514nm multiline (manual Laser Argon)
- 680-1080 nm (manual Laser Multiphotonen Chameleon)
XY Stage
Zeiss DC MCU 2008, motorized
- variable holder for slides and 3.5cm dishes