Light Microscopy

Zeiss

CZ6 - Zeiss LSM 880 Airy inverted

Category

Laser scanning confocal

Facility

LMF MPI-CBG

Location

, 038A

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Zeiss LSM 880 inverted single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2 PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition. 

Suitable for Specimen

  • Live samples
  • Fixed samples
  • Tissue sections

Suitable for Technique

  • 3D imaging
  • Large area tile/mosaic scans
  • Photo manipulation (PA/FRAP/FLIP/FLAP/...)

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Prior NanoScanZ

Objectives

  • Immersion:
    Air
  • Thread:
    M27
  • Zeiss Order Number:
    420639-9000-700
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9800-000
  • Immersion:
    W / Si / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0-0.17 mm
  • Working Distance:
    0.57 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 25/0,8 II (426947)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420852-9871
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421767-9970
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1022-818
  • Immersion:
    W / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.17 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 63/1,30 II HC (426958); LCI PN 63/1,30 III (426959)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420882-9970-720
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420782-9900-000

Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

Additional Filter(s)

Zeiss dichromatic mirror
  • (BP 420-460; LP 500), dichromatic mirror, longpass
  • LP 460, dichromatic mirror, longpass
  • LP 525, dichromatic mirror, longpass
  • LP 570, dichromatic mirror, longpass
  • LP 660, dichromatic mirror, longpass
  • SP 615, dichromatic mirror, shortpass

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120W (Zeiss, Metal halide lamp)
  • 355nm (Laser DPSS)
  • 405nm (integrated Laser Diode)
  • 458, 477, 488, 496, 514nm multiline (integrated Laser Argon)
  • 561nm (integrated Laser DPSS)
  • 594nm (integrated Laser HeNe)
  • 633nm (integrated Laser HeNe)

XY Stage

Märzhauser stage for Axio Observer, motorized
  • <link http://www.pecon.biz/wp-content/uploads/Flyer-Click-In-Premium_engl.pdf _blank external-link-new-window "Opens external link in new window">Pecon Click-In insert for 2x 35mm Petri Dishes</link>
  • <link http://www.pecon.biz/wp-content/uploads/Flyer-Click-In-Premium_engl.pdf _blank external-link-new-window "Opens external link in new window">Pecon Click-In insert for slides<br /></link>
  • heating insert P (Pecon)
  • heating insert M (Pecon)

Scan Head

scanner
Internal Detectors
  • 2 confocal PMT (spectral)
  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • AiryScan
  • T-PMT

Incubation

cage incubator (Zeiss)

Feature
Heating, Humidity, CO2

Additional Information

Getting started

Equipment

Publications