Light Microscopy

SD3 - Zeiss Spinning Disc

Category

Spinning disk

Facility

LMF MPI-CBG

Location

, 038B

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Real-Time Spinning Disc Confocal Microscope with DirectFRAP

Suitable for Specimen

  • Live samples
  • Organoids

Suitable for Technique

  • Multi wellplate imaging
  • Large area tile/mosaic scans

Microscope Stand

Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Prior NanoScanZ100

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420640-9800-000
  • Immersion:
    W / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0-0.17 mm
  • Working Distance:
    0.57 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 25/0,8 II (426947)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420852-9870
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.62 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    421867-9970
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.21 mm
  • Nomarski Prism:
    A; PA 40x/1,30 III (426965)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Order Number:
    420762-9800
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    421787-9970
  • Immersion:
    W / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.17 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 63/1,30 II HC (426958); LCI PN 63/1,30 III (426959)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420882-9970-720
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420782-9900-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.11 mm
  • Nomarski Prism:
    PA 100/1,46 III (426963)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Order Number:
    420792-9800-000

Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3, working distance 26 mm, motorized

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
  • BP 452/35; BP 525/30; BP 607/45; Transmission 100% (BP 435-470; BP 510-540; BP 585-630; Transmission 100%), triple dichromatic mirror, triple bandpass, Zeiss
  • empty

Additional Filter(s)

emission filter wheel (Switching Time 50 ms)
  • BP 450/50 (BP 425-475), emission filter, bandpass
  • LP 458, emission filter, longpass
  • BP 527/50; BP 645/60 (BP 502-552; BP 615-675), emission filter, double bandpass
  • BP 535/30 (BP 520-550), emission filter, bandpass
  • BP 629/62 (BP 598-660), emission filter, bandpass
  • BP 690/50 (BP 665-715), emission filter, bandpass

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • HXP 120C (120W, integrated Metal halide lamp)
  • 405nm (integrated Laser Diode)
  • 445nm (Laser DPSS)
  • 488nm (integrated Laser DPSS)
  • 520nm (Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 639nm (integrated Laser DPSS)

XY Stage

Märzhäuser Scanningtisch 130x100 STEP, motorized
  • <link http://www.pecon.biz/products/product/?pid=201%20%7C%20Universal%20Mounting%20Frame%20KM _blank external-link-new-window "Opens external link in new window">Pecon Universal Mounting Frame KM</link>
  • <link http://www.pecon.biz/wp-content/uploads/Flyer-Click-In-Premium_engl.pdf _blank external-link-new-window "Opens external link in new window">Pecon Click-In insert for 2x 35mm Petri Dishes</link>
  • <link http://www.pecon.biz/wp-content/uploads/Flyer-Click-In-Premium_engl.pdf _blank external-link-new-window "Opens external link in new window">Pecon Click-In insert for slides<br /></link>
  • heating insert P S1

Hardware Autofocus

Definite Focus.1

Scan Head

  • Maximum Spinning Disk Rotation Speed: 1500 - 5000 rpm
  • Scan Head Field of View Size: 10 mm x 7 mm
  • Physical Pinhole Diameter: 50 µm
  • Physical Pinhole Spacing: 250 µm

Photo Manipulation

Zeiss Direct FRAP 1

Photo Manipulation Modality
Aperture

Incubation

stage top incubator (<span style="font-size: 12px;">Temperature module S1 and CO2 module S1</span>)

Feature
CO2, Heating

Additional Equipment

Objective heater 

Additional Information

Getting started

Equipment

Publications