Light Microscopy

Andor

SD2 - Andor Spinning Disc FRAPPA

Category

Spinning disk

Facility

LMF MPI-CBG

Location

MPI-CBG, 038B

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW).

Microscope Stand

OLYMPUS IX 81, inverted stand, motorized objective revolver

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO10X2
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.25 mm
  • Working Distance:
    0.25 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UAPON40XW340
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLFLN60X
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.28 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO60XW
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.15 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPSLAPO60X0
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO100XO

Condenser

Ph2, NA 0.55, working distance 27 mm, manual

Fluorescence Filter Cubes

  • empty

Additional Filter(s)

Sutter emission filter wheel
  • BP 440/40; BP 521/21; BP 607/34; BP 700/45 (BP 420-460; BP 511-532; BP 590-624; BP 678-723), emission filter, quadro bandpass
  • LP 488 Razor edge, emission filter, longpass
  • BP 700/75, emission filter, bandpass
  • LP 568 Razor edge, emission filter, longpass
  • LP 635, emission filter, longpass
  • BP 445/45, emission filter, bandpass
  • empty
  • Polariser
  • BP 525/30, emission filter, bandpass
  • BP 607/36, emission filter, bandpass

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • HBO (100W, Mercury arc lamp)
  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 640nm (Laser DPSS)

Camera

  • Port, Magnification:
    Left side port
  • Sensor Size:
    8.98 x 6.71 mm
  • Maximum Frame Size:
    1392 x 1040 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    11 fps
  • Port, Magnification:
    Left side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Maximum Frame Size:
    512 x 512 dexels
  • Bit Depth:
    14 bit
  • Max Full Chip Frame Rate:
    35-549 fps

Additional Information

Getting started

Equipment

Publications