Light Microscopy

Andor

SD4 - Andor Revolution WD Borealis Mosaic

Category

Spinning disk

Facility

LMF MPI-CBG

Location

MPI-CBG, 036

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Real-Time Confocal Microscope Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination. Suitable for confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time.

Microscope Stand

OLYMPUS IX 83, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Prior NanoScanZ

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO10X2
  • Immersion:
    Silicone
  • Immersion:
    Silicone
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.3 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO60xS2
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO100XO
  • Immersion:
    Oil
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.19 mm
  • Working Distance:
    0.08 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UAPON150XOTIRF

Condenser

BF, DIC, Ph0, NA 0.55, working distance 27 mm, motorized

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • CoolLED pE-4000 (CoolLED, integrated LED)
  • 405nm (integrated Laser Diode)
  • 445nm (Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 514nm (Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 638nm (Laser DPSS)
  • 726nm (Laser DPSS)

Camera

  • Port, Magnification:
    left side port
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    1024 x 1024 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    26 fps
  • Port, Magnification:
    left side port
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    1024 x 1024 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    26 fps

XY Stage

Prior PRO SCAN III, motorized

Hardware Autofocus

OLYMPUS IX 81 ZDC

Scan Head

  • Maximum Spinning Disk Rotation Speed: 4.000 rpm
  • Maximum Scanning Speed: 200 fps
  • Scan Head Field of View Size: Variable aperture up to 19 mm x 19 mm
  • Physical Pinhole Diameter: 25µm, 50µm
  • Physical Pinhole Spacing: 250µm
  • Brightfield Bypass Mode

Photo Manipulation

Andor Mosaic, more information

Photo Manipulation Modality
DMD

Incubation

cage incubator (Pecon)

Feature
CO2, Heating, Humidity

Additional Information

Getting started

Equipment

Publications