Light Microscopy

Zeiss

LZ1 - Zeiss Lightsheet Z.1

Category

Light sheet

Facility

LMF MPI-CBG

Location

, 356

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Single Plane Illumination Microscope The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions. Suitable for Live imaging in 3D and 4D.

Suitable for Specimen

  • Live samples
  • Fixed samples
  • Organoids

Suitable for Technique

  • 3D imaging
  • Timelapse imaging

Microscope Stand

Zeiss LightSheet Z.1, special stand

Objectives

  • Immersion:
    Air
  • Thread:
    M27
  • Zeiss Order Number:
    400900-9010
  • Immersion:
    Air
  • Thread:
    M27
  • Zeiss Order Number:
    400900-9010
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    18.5 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420330-8210
  • Immersion:
    Air
  • Thread:
    M27
  • Immersion:
    Water
  • Thread:
    M27
  • Immersion:
    Water
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2.5 mm
  • Nomarski Prism:
    W PA 40/1,0 III (426967-9010)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421462-8210
  • Immersion:
    Water
  • Working Distance:
    2.1 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421480-8210

Fluorescence Filter Cubes

  • LP 490, dichromatic mirror
  • LP 505, emission filter
  • SP 490, emission filter
  • BP 595/40, emission filter, bandpass
  • LP 640, dichromatic mirror, longpass
  • LP 660, emission filter, longpass
  • LP 580, dichromatic mirror, longpass
  • BP 525/40, emission filter, bandpass
  • LP 660, emission filter, longpass
  • BP 480/40, emission filter, bandpass
  • LP 510, dichromatic mirror, longpass
  • BP 545/40, emission filter, bandpass
  • LP 560, dichromatic mirror, longpass
  • emission filter
  • LP 585, emission filter, longpass
  • mirror
  • BP 480/40, emission filter, bandpass
  • LP 510, dichromatic mirror, longpass
  • BP 545/40, emission filter, bandpass
  • BP 445/50, emission filter, bandpass
  • LP 490, dichromatic mirror
  • LP 660, emission filter, longpass
  • LP 640, dichromatic mirror, longpass
  • empty
  • LP 640, emission filter, longpass
  • LP 560, dichromatic mirror, longpass
  • BP 517/25, emission filter, bandpass
  • BP 595/40, emission filter, bandpass
  • LP 560, dichromatic mirror, longpass
  • emission filter
  • LP 585, emission filter, longpass
  • mirror

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • 405nm (integrated Laser Diode)
  • 445nm (Laser DPSS)
  • 488nm (integrated Laser DPSS)
  • 514nm (Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 638nm (Laser DPSS)

Camera

  • Port, Magnification:
    left side port
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    2048 x 2048 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    30 fps
  • Port, Magnification:
    top port
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    2048 x 2048 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    30 fps

Incubation

Sample chamber (Light sheet Z1 sample chamber, Zeiss, Zeiss)

Feature
CO2, Cooling, Heating, Humidity

Additional Information

Getting started

Equipment

Publications