Light Microscopy

NIKON

H2 - Nikon STORM TIRF

Category

TIRF, Widefield

Facility

LMF MPI-CBG

Location

MPI-CBG, 036

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Super resolution Microscope  Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers; Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.

Suitable for Technique

  • Superresolution Localisation Microscopy (PALM/STORM/...)

Microscope Stand

NIKON Eclipse Ti-E, inverted stand, motorized objective revolver, motorized z focus

Additional Magnification

1x / 1.5x manual zoom lens

Z Focus Device Additional

Mad City Labs Nano-Drive

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    4 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • NIKON Order Number:
    MRD00101
  • Immersion:
    W / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    - mm
  • Working Distance:
    0.35 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • NIKON Order Number:
    MRH07241
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.18 mm
  • Working Distance:
    0.22 mm
  • Nomarski Prism:
    60x II; 60x II IR
  • Thread:
    M27
  • Tube Length:
    ∞
  • NIKON Order Number:
    MRD07602
  • Immersion:
    Oil
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.22 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • NIKON Order Number:
    MRD01901
  • Immersion:
    Oil
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.20 mm
  • Working Distance:
    0.12 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • NIKON Order Number:
    MRY10059

Condenser

DIC I, DIC II, Polarizer, NA 0.52, working distance 30 mm, motorized

Fluorescence Filter Cubes

  • empty
  • BP 562/40, excitation filter, bandpass, Semrock
  • LP 593, dichromatic mirror, longpass, Semrock
  • BP 624/40 (BP 604-644), emission filter, bandpass, Semrock
  • BP 500/24 (BP 488-512), excitation filter, bandpass, Semrock
  • LP 520, dichromatic mirror, longpass, Semrock
  • BP 542/27 (BP 528-555), emission filter, bandpass, Semrock
  • BP 405/5; BP 489/4; BP 560/4; BP 637/14 (BP 400-410; 485-493; 555-564; 630-644), dichromatic mirror, quadro bandpass, Chroma
  • BP 405/5; BP 489/4; BP 560/4; BP 637/14 (BP 400-410; 485-493; 555-564; 630-644), dichromatic mirror, quadro bandpass, Chroma
  • BP 446/34; BP 523/42; BP 600/36; BP 677/28 (BP 430-462; BP 502-544; BP 582-617; BP 663-690), emission filter, quadro bandpass, Semrock
  • empty
  • BP 620/60 (BP 590-650), excitation filter, bandpass, Chroma
  • LP 660, dichromatic mirror, longpass, Chroma
  • BP 700/75, emission filter, bandpass, Chroma
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 660, dichromatic mirror, longpass, Chroma
  • BP 525/50, emission filter, bandpass, Chroma
  • BP 470/40, excitation filter, bandpass, Chroma
  • BP ~525/40; BP ~632/55 (BP 505-545; BP 605-660), dichromatic mirror, double bandpass, Chroma
  • BP 522/41; BP 632/62 (BP 501-542; BP 601-663), emission filter, double bandpass, Chroma
  • BP 406/15, excitation filter, bandpass, Semrock
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 457/50 (BP 432-482), emission filter, bandpass, Semrock
  • BP 510/40 (BP 490-530), emission filter, bandpass

Additional Filter(s)

Sutter emission filter wheel
  • BP 617/73, emission filter, bandpass, Semrock
  • LP 488 Razor edge, dichromatic mirror, longpass, Semrock
  • LP 664 RazorEdge, dichromatic mirror, longpass, Semrock
  • BP 447/60 (BP 417-477), emission filter, bandpass, AHF
  • empty
  • BP 512/23; BP 630/91 (BP 501-524; BP 585-676), emission filter, double bandpass, Semrock
  • BP 628/40 (BP 608-648), emission filter, bandpass, Semrock
  • BP 525/30 (BP 510-540), emission filter, bandpass, Semrock
  • BP 607/36 (BP 589-625), emission filter, bandpass, Semrock
  • BP 520/15 (BP 512-527), emission filter, bandpass, Semrock

Transmitted Illumination

  • (Nikon 100W Pillar Diascopic Illuminator)

Fluorescence Illumination

  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 640nm (Laser DPSS)
  • CoolLED pE-2 400/470/565/635 (CoolLED, integrated LED)
  • Metal Halide Lamp (130 W, NIKON, integrated Metal halide lamp)
  • TIRF Illuminator 405/488/561/640 (NIKON, integrated Laser)

Camera

  • Port, Magnification:
    Left side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Maximum Frame Size:
    512 x 512 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    56-11.074 fps
  • Port, Magnification:
    Right side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Maximum Frame Size:
    512 x 512 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    56-11.074 fps

XY Stage

Nikon Ti-S, motorized

Hardware Autofocus

NIKON Perfect Focus System

Incubation

cage incubator (Pecon)

Feature
CO2, Heating, Humidity

Additional Information

Getting started

Equipment

Publications