Light Microscopy

Zeiss

CZ7 - Zeiss LSM 880 Airy upright

Category

Laser scanning confocal

Facility

LMF MPI-CBG

Location

MPI-CBG, 038A

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Zeiss LSM 880 upright single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector.
Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition.

Suitable for Specimen

  • Fixed samples
  • Tissue sections
  • Live samples

Suitable for Technique

  • Spectral unmixing
  • Large area tile/mosaic scans

Microscope Stand

Zeiss Axio Imager.Z1, upright stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9800-000
  • Immersion:
    Water
  • Working Distance:
    2.6 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420957-9900
  • Immersion:
    Si / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.57 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 25/0,8 II (426947)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420852-9871
  • Immersion:
    W / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    - mm
  • Working Distance:
    0.21 – 0.17 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440541
  • Immersion:
    Water
  • Working Distance:
    2.5 mm
  • Nomarski Prism:
    W PA 40/1,0 II HC (426967-9020-000 ); W PA 40/1,0 III (426967-9010)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421462-9900-799
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421767-9970
  • Immersion:
    W / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.17 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 63/1,30 II HC (426958); LCI PN 63/1,30 III (426959)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420882-9970-720
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420782-9900-000

Condenser

H/DIC, Ph1, Ph2, Ph3, DF, DIC I, DIC II, DIC III, NA 0.9, working distance 1.2 mm, manual

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120W (Zeiss, Metal halide lamp)
  • 405nm (integrated Laser Diode)
  • 458, 477, 488, 514nm multiline (integrated Laser Argon)
  • 561nm (integrated Laser DPSS)
  • 594nm (integrated Laser HeNe)
  • 633nm (integrated Laser HeNe)

XY Stage

PIline Wienecke & Sinske GmbH M-686K008, motorized
  • universal disholder
  • heating insert P S1
  • holder for 1 slide

Scan Head

conventional scanner
Internal Detectors
  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • 2 confocal PMT (spectral)
  • AiryScan
  • T-PMT
  • Maximum Spinning Disk Rotation Speed: 1500 - 5000 rpm
  • Maximum Scanning Speed: 1000 fps
  • Scan Head Field of View Size: 10 mm x 7 mm
  • Physical Pinhole Diameter: 50 µm
  • Physical Pinhole Spacing: 250 µm

Photo Manipulation

Zeiss LSM 880, more information

Photo Manipulation Modality
Aperture

Incubation

stage top incubator (Bachhofer chamber, H. Saur)

Feature
Heating

Additional Information

Getting started

Equipment

Publications