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Light Microscopy

H3 - Andor STORM Spinning disc

Category

Spinning disk

Facility

LMF MPI-CBG

Location

, 340

Description

Real-Time Confocal Microscope Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser. Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

Suitable for Technique

  • Superresolution Localisation Microscopy (PALM/STORM/...)

Microscope Stand

Eclipse Ti, inverted stand, motorized objective revolver

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    4 mm
  • Thread:
    M27
  • Tube Length:
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.25 – 0.17 mm
  • Thread:
    M27
  • Tube Length:
  • Order Number:
    MRD00405
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.18 mm
  • Working Distance:
    0.27 mm
  • Thread:
    M27
  • Tube Length:
  • Order Number:
    MRD07602
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Thread:
    M27
  • Tube Length:
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.13 mm
  • Thread:
    M27
  • Tube Length:

Condenser

BF, DIC, Ph0, working distance 30 mm

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 561nm (integrated Laser DPSS)
  • 640nm (Laser DPSS)

Camera

  • Port, Magnification:
    left side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    56 fps

Scan Head

  • Maximum Spinning Disk Rotation Speed: 1500 - 10.000 rpm
  • Physical Pinhole Diameter: 25 µm
  • Physical Pinhole Spacing: 250 µm

Additional Information

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