Light Microscopy

OLYMPUS

W1 - Olympus TIRF

Category

TIRF, Widefield

Facility

LMF MPI-CBG

Location

MPI-CBG, U32

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Total Internal Reflection Microscope (TIRF) Olympus setup. Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics.

Suitable for Specimen

  • Molecules at the surface of a cover slip
  • Fixed samples
  • Live samples

Additional Magnification

Optovar 1x / 2x, manual

Objectives

  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO10X2
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Immersion:
    Special Oil
  • Coverslip Thickness:
    0.15 mm
  • Working Distance:
    0.08 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    APON100XHOTIRF
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.08 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UAPON150XOTIRF

Condenser

DIC, working distance 27 mm, manual

Fluorescence Filter Cubes

Additional Filter(s)

image splitter
  • SP 560, dichromatic mirror, shortpass
  • LP 590, dichromatic mirror, longpass
  • BP 520/15 (BP 512-527), emission filter, bandpass, Semrock
  • BP 525/30 (BP 510-540), emission filter, bandpass, Semrock
  • BP 617/73, emission filter, bandpass, Semrock
  • BP 628/40 (BP 608-648), emission filter, bandpass, Semrock
  • (BP 505-545; BP 575-745), emission filter wheel, double bandpass, Chroma

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • X-Cite 120W (X-Cite, Metal halide lamp)
  • 561nm (integrated Laser DPSS)
  • Laser DPSS 491nm (75mW, integrated Laser DPSS)
  • MPI-CBG W1 TIRF condenser 491nm/561nm (75mW, integrated Laser DPSS)

Camera

  • Port, Magnification:
    Left side port
  • Sensor Size:
    8.2 x 8.2 mm
  • Maximum Frame Size:
    512 x 512 dexels
  • Max Full Chip Frame Rate:
    56-11.074 fps
  • Port, Magnification:
    Top
  • Sensor Size:
    6 x 6 mm
  • Maximum Frame Size:
    1024x768 dexels
  • Bit Depth:
    8 bit

XY Stage

, manual

Incubation

(Bachhofer chamber, H. Saur)

Feature
Heating

Additional Information

Getting started

Equipment

Publications