Light Microscopy

Zeiss

W4 - Zeiss Inverted CCD

Category

Widefield

Facility

LMF MPI-CBG

Location

MPI-CBG, 341

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Wide-Field Microscope Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence Suitable for fixed samples, fluorescence, transmitted light, z stacks.

Suitable for Specimen

  • Fixed samples

Microscope Stand

Zeiss Axiovert 200M, inverted stand, motorized objective revolver

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    4 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440300
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    13.6 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440321
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    13.6 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440321
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1063-139
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0-1.5 mm
  • Working Distance:
    7.9 mm at cover glass 0.75 mm
  • Nomarski Prism:
    LD PN 20/0,40 II (426954)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441341-9970
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.61 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440649
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.71 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440350
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Nomarski Prism:
    40x/1.30 oil III (1033-912 )
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1022-819
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440460
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1018-595

Fluorescence Filter Cubes

  • BP 388/18 (BP 379-396), excitation filter, bandpass
  • LP 414, dichromatic mirror, longpass
  • BP 446/62 (BP 415-477), emission filter, bandpass
  • BP 469/40 (BP 449-488), excitation filter, bandpass
  • LP 495, dichromatic mirror, longpass
  • BP 514/30 (BP 495-532), emission filter, bandpass
  • BP 560/40 (BP 540-580), excitation filter, bandpass
  • LP 588, dichromatic mirror, longpass
  • BP 631/72 (BP 595-667), emission filter, bandpass
  • BP 706/84 (BP 664-748), dichromatic mirror, bandpass
  • BP 684/46 (BP 661-706), emission filter, bandpass

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HBO (100W, Mercury arc lamp)

Camera

  • Port, Magnification:
    top port
  • Sensor Size:
    11.1 x 7.9 mm
  • Maximum Frame Size:
    1520 x 1080 dexels
  • Bit Depth:
    8 bit
  • Max Full Chip Frame Rate:
    8.5-19 fps

Getting started

Equipment

Publications