Light Microscopy

Zeiss

W7 - Zeiss Laser Microdissection

Category

Microdissection, Widefield

Facility

LMF MPI-CBG

Location

MPI-CBG, U36

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Dissection Microscope a laser microdissection system based on an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color). Suitable for the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Laser cutting/ ablation

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    8.8 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420320-9901
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    12.5 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420130-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    1.9 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440135
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0-1.5 mm
  • Working Distance:
    7.9 mm at cover glass 0.75 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421350-9970
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0-1.5 mm
  • Working Distance:
    2.9 mm at cover glass 0.75 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421360-9970
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0-1.5 mm
  • Working Distance:
    1.7 mm at cover glass 0.75 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421380-9970
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420490-9900
  • Immersion:
    Glycerine
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.17 mm at cover glass 0.16 mm
  • Nomarski Prism:
    PA 150x/1,35 III (426968-9000)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420792-9970

Condenser

BF, DIC, Ph1, Ph2, Ph3, NA 0.55, working distance 26 mm, manual

Fluorescence Filter Cubes

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • 355nm (Laser Diode)
  • 365, 470, 590, 625 nm (Zeiss, integrated Colibri LED)
  • HXP 120C (120W, Zeiss, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    top port
  • Sensor Size:
    6.3 x 4.8 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    8 bit
  • Max Full Chip Frame Rate:
    15.38 fps
  • Port, Magnification:
    right side port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps

Photo Manipulation

Zeiss PALM MicroBeam

Photo Manipulation Modality
Scanner

Getting started

Equipment

Publications