Light Microscopy

Andor

L2 - Long Term Timelapse II

Category

Widefield

Facility

LMF MPI-CBG

Location

MPI-CBG, 240

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Wide-Field Microscope Motorized inverted wide-field system. Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.

Suitable for Specimen

  • protein droplets

Suitable for Technique

  • Multiple positions
  • Timelapse imaging

Microscope Stand

Zeiss Axiovert 200M, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    13.6 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440321
  • Immersion:
    Air
  • Coverslip Thickness:
    - mm
  • Working Distance:
    5.2 mm
  • Nomarski Prism:
    EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440331
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0-1.5 mm
  • Working Distance:
    10.2 mm at cover glass 1.0 mm
  • Nomarski Prism:
    LD 20x/0,40 epi+ (444447)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440845
  • Immersion:
    W / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    - mm
  • Working Distance:
    0.21 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440545
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.71 mm
  • Nomarski Prism:
    EC PN 40/0,75 II; 100/1,30III (426945)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440351
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    441757-9970
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0-1.5 mm
  • Working Distance:
    1.5 mm at cover glass 1.0 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440861
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    1031-171
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949); PA 100/1,40 III (426941)
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • Zeiss Order Number:
    440782-9902

Condenser

, NA 0.55, working distance 26 mm, motorized

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • CoolLED pE-4000 (CoolLED, integrated LED)

Camera

  • Port, Magnification:
    left port
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    2048 x 2048 dexels
  • Bit Depth:
    12 bit
  • Port, Magnification:
    top port
  • Sensor Size:
    22.18 x 22.18 mm
  • Maximum Frame Size:
    2016 x 2016 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    1279 fps

XY Stage

, motorized

Getting started

Equipment

Publications