Light Microscopy

[B CUBE] Ti-E with TIRF, STORM and Spinning Disk

Category

Super resolution, Widefield

Facility

MIM Facility CMCB

Location

, E22

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?sid=36

Description

tba

Suitable for Technique

  • Superresolution Localisation Microscopy (PALM/STORM/...)
  • 3D imaging
  • Large area tile/mosaic scans
  • Multi wellplate imaging
  • Multiple positions
  • Timelapse imaging

Microscope Stand

Eclipse Ti-E, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Mad City Labs Nano-Drive

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.25 – 0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.2 – 0.11 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.18 mm
  • Working Distance:
    0.31 – 0.28 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.18 – 0.16 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Collar:
    temperature and coverslip correction
  • Coverslip Thickness:
    0.13-0.20 mm
  • Working Distance:
    0.12 mm
  • Thread:
    M25
  • Tube Length:
    ∞

Condenser

, working distance 13 mm, manual

Transmitted Illumination

  • Metal halide lamp (Nikon D-LH/LC Halogen Lamp)

Fluorescence Illumination

  • Laser 405 nm (20 mW, integrated Laser)
  • Laser 488 nm (80 mW, integrated Laser)
  • Laser 561 nm (80 mW, integrated Laser)
  • Laser 647 nm (125 mW, integrated Laser)
  • Metal Halide Lamp (130 W, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    right side port, 1x
  • Sensor Size:
    13.3 x 13.3 mm
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    26 fps
  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.2 x 8.2 mm
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    56 fps
  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    5.7 x 4.3 mm
  • Maximum Frame Size:
    2592 x 1944 dexels
  • Bit Depth:
    8 bit
  • Max Full Chip Frame Rate:
    5.5 fps

XY Stage

Nikon TI-S-ER, motorized
  • holder for 1 slide
  • multiwell plate holder
  • petri dish holder
  • universal disholder

Hardware Autofocus

Perfect Focus System

Incubation

Stage inset (Peltier-based sample holder for slides or dishes)

Feature
Heating, Cooling

Additional Equipment

stage heating/cooling

Getting started

Equipment

Publications