Light Microscopy

[B CUBE] Ti-E with TIRF/FRAP

Category

TIRF, Widefield

Facility

MIM Facility CMCB

Location

, E22

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?sid=36

Description

tba

Suitable for Specimen

  • Cleared samples
  • Fixed samples
  • Live samples
  • Molecules at the surface of a cover slip
  • protein droplets
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans
  • Multi wellplate imaging
  • Multiple positions
  • Photo manipulation (PA/FRAP/FLIP/FLAP/...)
  • Timelapse imaging

Microscope Stand

Eclipse Ti-E, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    16 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.25 – 0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.18 – 0.16 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Collar:
    temperature and coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.12 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Collar:
    temperature and coverslip correction
  • Coverslip Thickness:
    0.13-0.20 mm
  • Working Distance:
    0.12 mm
  • Thread:
    M25
  • Tube Length:
    ∞

Condenser

, working distance 30 mm, manual

Transmitted Illumination

  • Metal halide lamp (Nikon D-LH/LC Halogen Lamp)

Fluorescence Illumination

  • Laser 405 nm (20 mW, integrated Laser)
  • Laser 488 nm (80 mW, integrated Laser)
  • Laser 561 nm (80 mW, integrated Laser)
  • Laser 647 nm (125 mW, integrated Laser)
  • SpectraX 395/25 nm LED (295 mW, integrated LED)
  • SpectraX 440/20 nm LED (256 mW, integrated LED)
  • SpectraX 470/24 nm LED (196 mW, integrated LED)
  • SpectraX 510/25 nm LED (62 mW, integrated LED)
  • SpectraX 550/15 nm LED (260 mW, integrated LED)
  • SpectraX 575/25 nm LED (310 mW, integrated LED)
  • SpectraX 640/30 nm LED (231 mW, integrated LED)

Camera

  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.2 x 8.2 mm
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    56 fps

XY Stage

Nikon TI-S-ER, motorized
  • holder for 1 slide
  • multiwell plate holder
  • petri dish holder
  • universal disholder

Hardware Autofocus

Perfect Focus System

Incubation

Objective heater (Objective Heater)

Feature
Heating

Additional Equipment

Objective Heater

Getting started

Equipment

Publications