Light Microscopy

Zeiss

DZNE W1 Zeiss ApoTome

Category

ApoTome, Widefield

Facility

LMF DZNE

Location

DZNE, 157

Link to Booking

https://techpool.biotec.tu-dresden.de/?scheduleid=sc14ffd3aa3e44c3

Description

Structured Illumination Microscope It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans

Microscope Stand

Zeiss Axio Imager.M2, upright stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    12.1 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420630-9900-000
  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9800-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.25 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420660-9970
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420490-9900

Condenser

BF, DIC, Ph1, Ph2, Ph3, NA 0.9, working distance 1.2 mm, manual

Fluorescence Filter Cubes

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
  • BP 546/12 (BP 540-552), excitation filter, bandpass, Zeiss
  • LP 560, dichromatic mirror, longpass, Zeiss
  • BP 607/65 (BP 575-640), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120C (120W, Zeiss, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    8.9 x 6.7 mm
  • Maximum Frame Size:
    1388 x 1040 dexels
  • Bit Depth:
    12 bit
  • Max Full Chip Frame Rate:
    12.86 fps
  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    8.7 x 6.6 mm
  • Maximum Frame Size:
    1936 x 1460 dexels
  • Bit Depth:
    14 bit
  • Max Full Chip Frame Rate:
    38 fps

XY Stage

Zeiss 130x85 STEP (D), motorized
  • holder for 1 slide

Structured Illumination

ApoTome2, structured illumination for optical sectioning

Getting started

Equipment

Publications