Light Microscopy

NIKON

[B CUBE] Ti-E with Spinning Disk

Category

Spinning disk

Facility

MIM Facility CMCB

Location

B CUBE, E22

Link to Booking

https://techpool.biotec.tu-dresden.de/schedule.php?sid=36

Description

tba

Suitable for Specimen

  • Cleared samples
  • Fixed samples
  • Live samples
  • Organoids
  • Tissue sections
  • Whole organisms
  • Whole organs

Suitable for Technique

  • 3D imaging
  • Large area tile/mosaic scans
  • Multi wellplate imaging
  • Multiple positions
  • Timelapse imaging

Microscope Stand

NIKON Eclipse Ti-E, inverted stand, motorized objective revolver, motorized z focus

Additional Magnification

1x / 1.5x manual zoom lens

Z Focus Device Additional

Mad City Labs Nano-Drive

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.25 – 0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.2 – 0.11 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.18 mm
  • Working Distance:
    0.31 – 0.28 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.15-0.19 mm
  • Working Distance:
    0.18 – 0.16 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Immersion:
    Oil
  • Collar:
    temperature and coverslip correction
  • Coverslip Thickness:
    0.13-0.20 mm
  • Working Distance:
    0.12 mm
  • Thread:
    M25
  • Tube Length:
    ∞

Condenser

, NA 0.72, working distance 13 mm, manual

Transmitted Illumination

  • Metal halide lamp (Nikon D-LH/LC Halogen Lamp)

Fluorescence Illumination

  • Laser 405 nm (20 mW, Agilent, integrated Laser)
  • Laser 488 nm (80 mW, Agilent, integrated Laser)
  • Laser 561 nm (80 mW, Agilent, integrated Laser)
  • Laser 647 nm (125 mW, Agilent, integrated Laser)

Camera

  • Port, Magnification:
    right side port, 1x
  • Sensor Size:
    13.3 x 13.3 mm
  • Maximum Frame Size:
    1024 x 1024 dexels
  • Bit Depth:
    16 bit
  • Max Full Chip Frame Rate:
    26 fps

XY Stage

Nikon TI-S-ER, motorized
  • holder for 1 slide
  • multiwell plate holder
  • petri dish holder
  • universal disholder

Hardware Autofocus

NIKON Perfect Focus System

Scan Head

  • Maximum Spinning Disk Rotation Speed: 1500 - 5000 rpm
  • Maximum Scanning Speed: 1000 fps
  • Scan Head Field of View Size: 10 mm x 7 mm
  • Physical Pinhole Diameter: 50 µm
  • Physical Pinhole Spacing: 253 µm

Incubation

Stage inset (Peltier-based sample holder for slides or dishes, Warner Instruments)

Feature
Heating, Cooling

Additional Equipment

stage heating/cooling

Getting started

Equipment

Publications