Light Microscopy
MIM Facility
[B CUBE] iSCAT
Category
Interferometric Scattering Detection (iSCAT), Widefield
Facility
MIM Facility CMCB
Location
B CUBE,
E23
Link to Booking
Description
The interferometric scattering (iSCAT) microscope of the MIM Facility at B CUBE is a home-built microscope offering the possibility of label-free detection and tracking of biomolecules. To improve SNR and thus recording speed and localization precision, gold nanoparticles can be used as labels with radii as low as a few nm -- thus providing a huge advantage over the classical dark-field-scattering approach.
Since the move of B CUBE into the new building at Tatzberg 41, we are still wating for the companies to complete the room for the iSCAT setup -- but we are happy to discuss your projects and ideas with you already; just contact us.
Suitable for Specimen
- Molecules at the surface of a cover slip
- protein droplets
Microscope Stand
homebuilt inverted, inverted stand, manual objective revolver, motorized z focus
Objectives
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Immersion:Oil
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Coverslip Thickness:0.17 mm
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Working Distance:0.13 mm
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Thread:RMS (20.32×0.71 mm)
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Tube Length:∞
Fluorescence Illumination
- Laser 488 nm (100 mW, Toptica, manual Diode Laser)
- Laser 520 nm (1 W, Lasertack, manual Diode Laser)
- Laser 635 nm (700 mW, Lasertack, manual Diode Laser)
Camera
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Sensor Size:10.9 x 10.9 mm
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Maximum Frame Size:1024 x 1024 dexels
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Bit Depth:12 bit
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Max Full Chip Frame Rate:150 fps