Light Microscopy

Leica

SP8 MP inverse BIOTEC

Category

Multi photon, Laser scanning confocal

Facility

LMF CMCB

Location

BIOTEC, 2nd floor, 225

Link to Booking

https://techpool.biotec.tu-dresden.de/index.php?redirect=%2Fschedule.php%3F%26

Description

It is an inverted Laser Scanning Confocal Microscope with a pulsed 405nm laser, a pulsed white light laser and a dual beam Insight X3 pulsed multiphoton laser. It offers five internal spectral HyD detectors, one transmission channel and a spectral NDD detection unit with four HyD detectors. Additionally it provides an independent four laser line photomanipulation unit (RAPP, 405, 488, 561, 640nm) and incubation for live cell imaging. Besides the conventional scanner it supplies a resonant scanner (8.000 Hz). High resolution imaging is supported by the integrated deconvolution (lightning). It is suitable for optical sectioning, intensity imaging (with gating for autofluorescence removal), deep tissue imaging and especially fast fluorescence lifetime microscopy (FLIM). , deep tissue imaging, photo-manipulation and high speed scanning.

Suitable for Specimen

  • Live samples
  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Multi wellplate imaging
  • Multiple positions
  • Timelapse imaging
  • Photo manipulation (PA/FRAP/FLIP/FLAP/...)
  • 3D imaging
  • FLIM
  • Large area tile/mosaic scans

Microscope Stand

Leica DMI 8, inverted stand, motorized objective revolver, motorized z focus

Z Focus Device Additional

Leica SuperZ stage

Objectives

  • Immersion:
    Air
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506407
  • Immersion:
    W / Si / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506533
  • Immersion:
    W / Si / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506343
  • Immersion:
    Water
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506357
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506351
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506346
  • Immersion:
    Oil
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.17 mm
  • Thread:
    M25
  • Tube Length:
    ∞
  • Leica Order Number:
    15506353

Condenser

BF, NA 0.55, working distance 28 mm, motorized

Fluorescence Filter Cubes

  • BP 350/50 (BP 325-375), excitation filter, bandpass, Leica
  • LP 409, dichromatic mirror, longpass, Leica
  • BP 460/50 (BP 435-485), emission filter, bandpass, Leica
  • BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
  • LP 505, dichromatic mirror, longpass, Leica
  • BP 527/30 (BP 512-542), emission filter, bandpass, Leica
  • BP 546/12 (BP 540-552), excitation filter, bandpass, Leica
  • LP 560, dichromatic mirror, longpass, Leica
  • BP 585/40 (BP 565-605), emission filter, bandpass, Leica
  • DM 80/20, double dichromatic mirror, mirror, Leica
  • SP 670, dichromatic mirror, mirror, Leica
  • SP 750, dichromatic mirror, mirror, Leica
  • SP 795, dichromatic mirror, mirror, Leica

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • HXP 120C (120W, Leica, integrated Metal halide lamp)
  • 405nm pulsed (manual Laser Diode)
  • pulsed White Light Laser (WLL) - 470-670 nm (1 mW, integrated Laser)
  • tunable 680 - 1300 nm, fixed 1045 nm (Spectra Physics, integrated Laser Multiphotonen)

XY Stage

Märzhäuser Type, SN: 499985, BZ:07, motorized
  • variable holder for slides and 3.5cm dishes
  • multiwell plate holder

Scan Head

conventional+resonant scanner, 8 kHz
Internal Detectors
  • 1 spectral detection PMT
  • 2 spectral detection HyD (gated)
  • 2 spectral detection SMD (HyD)
  • T-PMT
Additional Detectors
  • 4 Tune 4-channel HyD RLD

Photo Manipulation

RAPP UGA42 Firefly

Photo Manipulation Modality
Scanner

Incubation

splitable cage incubator, black (Life Imaging Service)

Feature
CO2, Heating, Humidity

Additional Equipment

An InsightX3 laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes. A resonating scanner allows fast confocal imaging. The 4-Tune unit is enables FLIM measurements.

Computer

  • OS:
    Windows 10
  • CPU:
    Intel Xeon Gold 6134 3,2GHz
  • RAM:
    192 GB
  • Graphics Card:
    NVIDIA Quadro GP100 (16GB, 3584 kernels)
  • Display(s):
    2 x 32"
Software Package(s)
Leica LAS X SP8
Module(s): 3D Visualisation, Colocalization, Dye Finder, HCS A Developer SP8, Lightning Expert, Live Data Mode, MicroLab, SP8 Controle Software

Getting started

Equipment

Publications