Light Microscopy

OLYMPUS

CO1 - Olympus Fluoview 1000

Category

Single Photon, Laser scanning confocal

Facility

LMF MPI-CBG

Location

MPI-CBG, 038A

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Single photon point scanning, Olympus FV1000 confocal microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser.

Suitable for Specimen

  • Fixed samples
  • Live samples

Suitable for Technique

  • Photo manipulation (PA/FRAP/FLIP/FLAP/...)
  • Laser cutting/ ablation
  • Multi wellplate imaging

Microscope Stand

OLYMPUS IX 81, inverted stand, motorized objective revolver, motorized z focus

Additional Magnification

1/1.6x

Objectives

  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    3.1 mm
  • Nomarski Prism:
    A
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    11506038
  • Immersion:
    Oil
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    A
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO20XO
  • Immersion:
    Oil
  • Collar:
    NA correction
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.1 mm
  • Nomarski Prism:
    A
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UAPON 40XO340-2
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.11-0.23 mm
  • Working Distance:
    0.18 mm
  • Nomarski Prism:
    A
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO40X
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.28 mm
  • Nomarski Prism:
    A
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO60XW
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.15 mm
  • Nomarski Prism:
    A
  • Thread:
    RMS (20.32×0.71 mm)
  • Tube Length:
    ∞
  • OLYMPUS Order Number:
    UPLSAPO60XO

Condenser

BF, DIC, NA 0.55, motorized

Fluorescence Filter Cubes

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • X-Cite 120W (X-Cite, Metal halide lamp)
  • 458, 477, 488, 514nm multiline (manual Laser Argon)
  • 375nm (integrated Laser Diode)
  • 405nm (manual Laser Diode)
  • 405nm pulsed (manual Laser Diode)
  • 561nm (manual Laser DPSS)
  • 633nm (manual Laser HeNe)

XY Stage

Prior, motorized
  • heating insert P-Set 2000 (Pecon)
  • multiwell plate holder
  • holder for 1 slide
  • petri dish holder

Scan Head

conventional scanner
Internal Detectors
  • 2 confocal PMT (filter)
  • T-PMT

Photo Manipulation

Fluoview FV1000 ASU

Photo Manipulation Modality
Scanner

Incubation

stage top incubator (Pecon)

Feature
Heating, CO2

Additional Information

Getting started

Equipment

Publications