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Light Microscopy

Zeiss

DZNE C2 - Zeiss LSM 980 Airy inverted

Category

Laser scanning confocal

Facility

LMF DZNE

Location

, 253

Description

Zeiss LSM 880 inverted single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2 PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • 3D imaging
  • Large area tile/mosaic scans
  • Photo manipulation (PA/FRAP/FLIP/FLAP/...)

Microscope Stand

Zeiss Axio Observer 7 SP, inverted stand, motorized z focus

Z Focus Device Additional

PI Z-Piezo WSB 500

Condenser

BF, Ph1, Ph2, Ph3, DIC II, DIC III, NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

Additional Filter(s)

Zeiss emission filter wheel
  • (BP 420-480; BP 570-630), double bandpass
  • (BP 465-505; BP 525-585), double bandpass
  • (BP 495-550; BP 570-630), double bandpass
  • (BP 495-560; LP 660), emission filter, bandpass + longpass
  • (BP 570-620; LP 660), bandpass + longpass

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • 630, 590, 555, 475, 385nm (Zeiss, integrated Colibri 7 LEDs)

XY Stage

PI scanning stage 130x100 PIEZO, motorized
  • mounting frame Z-Piezo WSB Universal
  • mounting frame z-piezo WSB multiwell plates

Scan Head

scanner
Internal Detectors
  • AiryScan 2
  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • 2 confocal PMT (spectral)
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