Light Microscopy

Zeiss

W9 - Zeiss Upright Apotome

Category

ApoTome, Widefield

Facility

LMF MPI-CBG

Location

, 036

Description

Research-grade upright widefield microscope equipped with a motorized stage and an ApoTome.2 for improved z-contrast. The system features two cameras: one color camera for Brightfield imaging of e.g. histological samples and one B/W camera for Differential Interference Contrast (DIC), Darkfield and Fluorescence imaging. Image pixel sizes

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • 3D imaging
  • Large area tile/mosaic scans

Microscope Stand

Zeiss Axio Imager.Z2, upright stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.55 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420650-9902-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.25 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420660-9970-000
  • Immersion:
    Air
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.13-0.21 mm
  • Working Distance:
    0.12 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420380-9970-000
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.17 mm
  • Nomarski Prism:
    PA 100/1,4 HC II (426949)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420792-9901-000

Condenser

BF, DF, DIC II, DIC III, Ph1, Ph2, Ph3, NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

  • BP 500/24 (BP 488-512), excitation filter, bandpass, Zeiss
  • LP 515, dichromatic mirror, longpass, Zeiss
  • BP 535/30 (BP 520-550), emission filter, Zeiss
  • BP 436/25 (BP 424-449), excitation filter, bandpass, Zeiss
  • LP 455, dichromatic mirror, longpass, Zeiss
  • BP 480/40 (BP 460-500), emission filter, bandpass, Zeiss
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

Transmitted Illumination

  • LED (LED)

Fluorescence Illumination

  • HXP 120V (120W, Zeiss, integrated Metal halide lamp)

Camera

  • Port, Magnification:
    top port, 1x
  • Sensor Size:
    12 x 10 mm
  • Maximum Frame Size:
    2752 x 2208 dexels
  • Bit Depth:
    14 bit
  • Max Full Chip Frame Rate:
    19 fps
  • Port, Magnification:
    left side port, 1x
  • Sensor Size:
    13.2 x 8.8 mm
  • Maximum Frame Size:
    4250 x 2838 dexels
  • Bit Depth:
    14 bit
  • Max Full Chip Frame Rate:
    10 fps

XY Stage

Maerzhaeuser Scanning Stage 130x85 STEP, motorized
  • holder for 1 slide

Structured Illumination

ApoTome2, structured illumination for optical sectioning

Computer

  • OS:
    Windows 10
  • CPU:
    Intel Xeon E5-2623 v3 (2x)
  • RAM:
    256 GB
  • Graphics Card:
    NVIDIA Quadro M4000
  • Display(s):
    32"
Software Package(s)
Zeiss Zen Blue 2.6 2.6

Getting started

Equipment

Publications