Light Microscopy

Zeiss

CZ5 - Zeiss LSM 700 inverted

Category

Single Photon, Laser scanning confocal

Facility

LMF MPI-CBG

Location

MPI-CBG, 038A

Link to Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Description

Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ.The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Suitable for Specimen

  • Fixed samples
  • Tissue sections

Suitable for Technique

  • Large area tile/mosaic scans

Microscope Stand

Zeiss Axio Observer.Z1, inverted stand, motorized objective revolver, motorized z focus

Objectives

  • Immersion:
    Air
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    2 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420640-9800-000
  • Immersion:
    W / Glyc / Oil
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0-0.17 mm
  • Working Distance:
    0.21 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 25/0,8 II (426947)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420852-9972
  • Immersion:
    Water
  • Collar:
    coverslip correction
  • Coverslip Thickness:
    0.14-0.19 mm
  • Working Distance:
    0.28 mm at cover glass 0.17 mm
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    421767-9970
  • Immersion:
    W / Glyc
  • Collar:
    immersion correction
  • Coverslip Thickness:
    0.15-0.18 mm
  • Working Distance:
    0.17 mm at cover glass 0.17 mm
  • Nomarski Prism:
    LCI PN 63/1,30 II HC (426958); LCI PN 63/1,30 III (426959)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420882-9970-720
  • Immersion:
    Oil
  • Coverslip Thickness:
    0.17 mm
  • Working Distance:
    0.19 mm
  • Nomarski Prism:
    PA 63/1,4 HC II (426952); PA 63/1,40 III (426957)
  • Thread:
    M27
  • Tube Length:
    ∞
  • Zeiss Order Number:
    420782-9900-000

Fluorescence Filter Cubes

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 510, dichromatic mirror, longpass, Zeiss
  • BP 540/50 (BP 515-565), emission filter, bandpass, Zeiss
  • BP 546/12 (BP 540-552), excitation filter, bandpass, Zeiss
  • LP 560, dichromatic mirror, longpass, Zeiss
  • BP 607/65 (BP 575-640), emission filter, bandpass, Zeiss

Transmitted Illumination

  • Lamp (Halogen, 100W)

Fluorescence Illumination

  • HXP 120W (Zeiss, Metal halide lamp)
  • 405nm (integrated Laser Diode)
  • 488nm (integrated Laser DPSS)
  • 555nm (integrated Laser DPSS)
  • 639nm (integrated Laser DPSS)

XY Stage

Märzhauser Wetzler GmbH EK 130X85 mot. Tango CZ EMV, motorized
  • universal disholder
  • multiwell plate holder

Scan Head

conventional scanner
Internal Detectors
  • 2 confocal PMT (filter)

Photo Manipulation

Zeiss LSM 700

Photo Manipulation Modality
Scanner

Additional Information

Getting started

Equipment

Publications